FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology. Described is a group of inventions involving a method of determining TNFR2:Fc with a disulphide bond Cys78-Cys88 in sample containing TNFR2:Fc with disulphide bonds Cys74-Cys88/Cys78-Cys96 and TNFR2:Fc with disulphide bond Cys78-Cys88, method of purifying TNFR2: Fc with disulphide bonds Cys74-Cys88/ Cys78-Cys96, method of producing and purifying TNFR2:Fc with disulphide Cys bonds74-Cys88/Cys78-Cys96 and TNFR2:Fc with disulphide bond Cys78-Cys88 and a composition of TNFR2:Fc for treating a disease selected from ankylosing spondylitis, juvenile rheumatoid arthritis, psoriasis, psoriatic arthritis and rheumatoid arthritis. In one embodiment, the method of producing and purifying TNFR2:Fc involves a step for preparing a composition containing TNFR2:Fc with disulphide Cys74-Cys88/Cys78-Cys96 and TNFR2:Fc with disulphide bond Cys78-Cys88.
EFFECT: invention extends the range of methods for determining and purifying TNFR2:Fc in a sample.
15 cl, 7 dwg, 7 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| VERSIONS OF PERTUZUMAB AND THEIR ANALYTICAL CHARACTERISTICS | 2014 |
|
RU2737727C2 |
| HETERODIMERIC BISPECIFIC ANTIBODY AGAINST TNFα/AGAINST IL-17A, SIMILAR BY STRUCTURE TO NATURAL ANTIBODY, AND ITS PRODUCTION METHOD | 2019 |
|
RU2781816C1 |
| VERSIONS AND ISOFORMS OF ANTIBODIES WITH REDUCED BIOLOGICAL ACTIVITY | 2018 |
|
RU2813990C2 |
| METHODS AND COMPOSITIONS INCLUDING PURIFIED RECOMBINANT POLYPEPTIDES | 2014 |
|
RU2793783C1 |
| ANTI-PD-L1/ANTI-4-1BB BISPECIFIC ANTIBODY IN HETERODIMERIC FORM, SIMILAR IN STRUCTURE TO NATURAL ANTIBODY, AND PRODUCTION THEREOF | 2022 |
|
RU2825838C1 |
| BIOPHARMACEUTICAL COMPOSITIONS AND METHODS RELATED THERETO | 2020 |
|
RU2824761C2 |
| PROTEIN COMPOSITIONS AGAINST VEGF AND METHODS FOR THEIR PRODUCTION | 2020 |
|
RU2795973C1 |
| Fc FUSION HIGH AFFINITY IgE RECEPTOR α-CHAIN | 2016 |
|
RU2715606C2 |
| PROTEIN COMPOSITIONS AGAINST VEGF AND METHODS FOR THEIR PREPARATION | 2020 |
|
RU2788949C1 |
| CHIMERIC PROTEIN COMPOSED OF AN NGF ANTAGONIST DOMAIN AND A TNFΑ ANTAGONIST DOMAIN | 2015 |
|
RU2788122C2 |
