FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology. Invention is a test system for detecting DNA of an ornithosis agent (Chlamydophila psittaci) in birds, comprising plastic bottles and test tubes, a thermostable Tag-polymerase enzyme, a reaction-setting buffer, a mixture of four deoxynucleotide triphosphates, an internal control sample, a positive control sample, a recombinant plasmid containing a gene fragment of an ornithosis agent (Chlamydophila psittaci), synthetic oligonucleotide primers and dyes labeled with dyes, according to the invention, for the internal control sample, a bacteriophage T4 suspension with concentration of 5×103 copies of nucleotide sequences per 1 mcl, and for a positive control sample – a mixture of recombinant plasmid DNA containing a fragment of the genome of ornithosis DNA (Chlamydophila psittaci) and a fragment of the bacteriophage T4 genome, taken in ratio 1:1.
EFFECT: invention enables authentically diagnosing an ornithosis DNA exciter (Chlamydophila psittaci) in birds.
1 cl, 3 dwg, 4 tbl
Title | Year | Author | Number |
---|---|---|---|
METHOD FOR DETECTING DNA OF AVNITHOSIS AGENT (CHLAMYDOPHILA PSITTACI) IN BIRDS | 2018 |
|
RU2700456C1 |
TEST SYSTEM FOR DETECTING CHLAMYDIAL DNA IN LIVESTOCK ANIMALS AND BIRDS | 2018 |
|
RU2701332C1 |
METHOD FOR DETECTING DNA OF CHLOMYDIA IN FARM ANIMALS AND BIRDS | 2018 |
|
RU2700381C1 |
TEST SYSTEM FOR DETECTING SALMONELLA (SALMONELLA SPP.) DNA IN ANIMAL BIOLOGICAL MATERIAL, FOOD PRODUCTS AND ANIMAL AND VEGETABLE FODDERS | 2018 |
|
RU2700247C1 |
METHOD FOR DETECTING SALMONELLA (SALMONELLA SPP) DNA IN BIOLOGICAL MATERIAL OF ANIMALS, FOOD AND ANIMAL AND VEGETABLE FODDERS | 2018 |
|
RU2700476C1 |
TEST SYSTEM FOR DETECTING THE GENOME OF THE CAUSATIVE AGENT OF BORDETELLA BRONCHISEPTICA DNA IN FARM LIVESTOCK | 2018 |
|
RU2700477C1 |
TEST SYSTEM FOR DETECTING DNA OF LUMPY SKIN DISEASE VIRUS (LSDV) IN BIOLOGICAL MATERIAL OF ANIMALS USING A POLYMERASE CHAIN REACTION IN REAL TIME | 2019 |
|
RU2726242C1 |
TEST SYSTEM FOR DETECTING DNA OF RHINOTRACHEITIS VIRUS (BOVINE HERPES VIRUS 1, BOHV-1) IN CATTLE | 2018 |
|
RU2700254C1 |
METHOD FOR DETECTION OF DNA OF THE BORDETLELOSIS (BORDETELLA BRONCHISEPTICA) AGENT GENOME IN FARM ANIMALS | 2018 |
|
RU2703405C1 |
TEST SYSTEM FOR DETERMINING DNA OF NODULAR DERMATITIS VIRUS (LSDV) IN BIOLOGICAL MATERIAL OF ANIMALS BY PCR WITH ELECTROPHORETIC DETECTION OF AMPLIFICATION PRODUCTS IN AGAROSE GEL | 2019 |
|
RU2726432C1 |
Authors
Dates
2019-09-17—Published
2018-10-01—Filed