TEST SYSTEM FOR DETECTING DNA OF BIRD FLU ORNITHOSIS (CHLAMYDOPHILA PSITTACI) IN BIRDS Russian patent published in 2019 - IPC C12Q1/68 

Abstract RU 2700448 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology. Invention is a test system for detecting DNA of an ornithosis agent (Chlamydophila psittaci) in birds, comprising plastic bottles and test tubes, a thermostable Tag-polymerase enzyme, a reaction-setting buffer, a mixture of four deoxynucleotide triphosphates, an internal control sample, a positive control sample, a recombinant plasmid containing a gene fragment of an ornithosis agent (Chlamydophila psittaci), synthetic oligonucleotide primers and dyes labeled with dyes, according to the invention, for the internal control sample, a bacteriophage T4 suspension with concentration of 5×103 copies of nucleotide sequences per 1 mcl, and for a positive control sample – a mixture of recombinant plasmid DNA containing a fragment of the genome of ornithosis DNA (Chlamydophila psittaci) and a fragment of the bacteriophage T4 genome, taken in ratio 1:1.

EFFECT: invention enables authentically diagnosing an ornithosis DNA exciter (Chlamydophila psittaci) in birds.

1 cl, 3 dwg, 4 tbl

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RU 2 700 448 C1

Authors

Kotelnikova Aleksandra Andreevna

Malyshev Denis Vladislavovich

Bannov Vasilij Aleksandrovich

Chernykh Oleg Yurevich

Egorov Ivan Afanasevich

Drobin Yurij Dmitrievich

Dzhavadov Eduard Dzhavadovich

Donnik Irina Mikhajlovna

Saleeva Irina Pavlovna

Lysenko Aleksandr Anatolievich

Tyurin Vladimir Grigorevich

Krivonos Roman Anatolevich

Loretts Olga Gennadevna

Shevkoplyas Vladimir Nikolaevich

Deltsov Aleksandr Aleksandrovich

Koshchaev Andrej Georgievich

Lysenko Yurij Andreevich

Dajbova Lyubov Anatolevna

Dates

2019-09-17Published

2018-10-01Filed