TEST SYSTEM FOR DETECTING THE GENOME OF THE CAUSATIVE AGENT OF BORDETELLA BRONCHISEPTICA DNA IN FARM LIVESTOCK Russian patent published in 2019 - IPC C12Q1/68 

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology. Invention is a test system for DNA detection of genome of Brucella spp infection agent in farm livestock by means of polymerase chain reaction in real time, including plastic bottles and test tubes, a thermostable enzyme Tag-polymerase, a reaction buffer, a mixture of four deoxynucleotide triphosphates, an internal control sample, a positive control – a recombinant plasmid containing a fragment of the Brucella spp gene, synthetic oligonucleotide primers and probes marked with dyes, according to the invention for the internal control sample a bacteriophage T4 suspension with concentration of 5×10³ copies of nucleotide sequences per 1 mcl, and for a positive control sample – a mixture of recombinant plasmid DNA containing a fragment of the DNA genome Brucella spp and a fragment of the bacteriophage T4 genome, taken in ratio 1:1.

EFFECT: invention enables reliable diagnosing of Bordetella bronchiseptica DNA exciter.

1 cl, 2 dwg, 4 tbl