TEST SYSTEM FOR DETERMINING DNA OF NODULAR DERMATITIS VIRUS (LSDV) IN BIOLOGICAL MATERIAL OF ANIMALS BY PCR WITH ELECTROPHORETIC DETECTION OF AMPLIFICATION PRODUCTS IN AGAROSE GEL Russian patent published in 2020 - IPC C12Q1/68 

FIELD: biotechnology.

SUBSTANCE: invention represents a test system for detecting DNA of nodular dermatitis virus (LSDV) in biological material of animals by PCR method with electrophoretic detection of amplification products in agarose gel, including PCR buffer, a mixture thereof, consisting of deoxynucleoside triphosphates, oligonucleotide primers specific for the genome region of the causative agent of the infectious disease and for an internal control sample in the form of a bacteriophage T4 suspension with concentration of 5×10³ phagene particles per 1 mcl; a mixture of enzymes from DNA polymerase with enzyme inhibiting enzymes, TAQPOLYMERASE, a positive control sample which is a mixture of recombinant plasmid DNA containing a genome fragment of an infectious disease agent and a fragment of the bacteriophage T4 genome, taken in volume ratio of 1:1, according to the invention, fragments of bacteriophage T4 genomes and a nodular dermatitis virus (LSDV) genome fragment with the following nucleotide sequences are used for the positive control sample. To expand functional capabilities, high specificity when observing residual amounts of unknown DNA molecules of nodular dermatitis virus and reduced cost of the method.

EFFECT: invention broadens functional capabilities, increases specificity when detecting residual quantities of unknown DNA molecules of nodular dermatitis virus.

1 cl, 6 tbl