FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology. Invention is represented by a dense nutrient medium for Brucella cultivation, including mixing dry fermentative peptone, sodium chloride, microbiological agar, mixed hydrolyzate of embryo and extraembryonic birds tissues – stimulator C with distilled water, heating to boiling, boiling till complete agar melting, filtration, pH adjustment with hydrochloric acid solution (1:1) to pH 7.2±0.1, pouring finished filtrate into flasks and hermetic sealing, sterilization in autoclave.
EFFECT: use of the given medium enables to grow a sufficient number of colonies of the brucellosis agent early in 24–48 hours.
1 cl, 3 ex
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Authors
Dates
2020-07-29—Published
2019-11-25—Filed