NUTRIENT MEDIUM DENSE FOR BRUCELLA CULTIVATION Russian patent published in 2020 - IPC A61K35/30 C12N1/00 

Abstract RU 2728379 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology. Invention is represented by a dense nutrient medium for Brucella cultivation, including mixing dry fermentative peptone, sodium chloride, microbiological agar, mixed hydrolyzate of embryo and extraembryonic birds tissues – stimulator C with distilled water, heating to boiling, boiling till complete agar melting, filtration, pH adjustment with hydrochloric acid solution (1:1) to pH 7.2±0.1, pouring finished filtrate into flasks and hermetic sealing, sterilization in autoclave.

EFFECT: use of the given medium enables to grow a sufficient number of colonies of the brucellosis agent early in 24–48 hours.

1 cl, 3 ex

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RU 2 728 379 C1

Authors

Katunina Lyudmila Semenovna

Kulichenko Aleksandr Nikolaevich

Kurilova Anna Alekseevna

Kovtun Yurij Sergeevich

Timchenko Lyudmila Dmitrievna

Rzhepakovskim Igor Vladimirovich

Sizonenko Marina Nikolaevna

Avanesyan Svetlana Surenovna

Vakulin Valerij Nikolaevich

Safonnikova Viktoriya Gennadevna

Vasilenko Ekaterina Igorevna

Zhilchenko Elena Borisovna

Serdyuk Nataliya Sergeevna

Konyaeva Olga Anatolevna

Belozerova Olga Nikolaevna

Zharinova Nina Vadimovna

Dates

2020-07-29Published

2019-11-25Filed