FIELD: biotechnology; medicine; agriculture.
SUBSTANCE: invention refers to genetic engineering and can be used in biotechnology, medicine and agriculture to develop gene therapy preparations. Presented is a gene-therapeutic DNA vector based on a gene-therapeutic DNA vector VTvaf17, carrying a target gene selected from a group of genes NOS2, NOS3, VIP, KCNMA1, CGRP, to increase expression level of this target gene in human body and animals. Gene-therapeutic DNA vector VTvaf17-NOS2, or VTvaf17-NOS3, or VTvaf17-VIP, or VTvaf17-KCNMA1, or VTvaf17-CGRP has a nucleotide sequence SEQ ID No. 1 or SEQ ID No. 2 or SEQ ID No. 3 or SEQ ID No. 4 or SEQ ID No. 5, respectively. Invention also discloses a method of producing said vector, use of a vector, an Escherichia coli strain carrying said vector, as well as a method of producing said vector on an industrial scale.
EFFECT: invention can be used for safe application for human and animal genetic therapy.
16 cl, 22 ex, 16 dwg
