GENE-THERAPEUTIC DNA-VECTOR BASED ON GENE-THERAPEUTIC DNA-VECTOR VTVAF17, CARRYING TARGET GENE SELECTED FROM SHH, CTNNB1, NOG, WNT7A GENE GROUP TO INCREASE LEVEL OF EXPRESSION OF THESE TARGET GENES, METHOD FOR PRODUCTION AND USE THEREOF, STRAIN ESCHERICHIA COLI SCS110-AF/VTVAF17-SHH, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-CTNNB1, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-NOG, OR ESCHERICHIA COLI SCS110-AF/VTVAF17-WNT7A, CARRYING GENE-THERAPEUTIC DNA VECTOR, METHOD FOR PRODUCTION THEREOF, METHOD FOR INDUSTRIAL PRODUCTION OF GENE-THERAPEUTIC DNA VECTOR Russian patent published in 2020 - IPC C12N15/12 C12N15/70 C12N1/21 A61K48/00 C12R1/19 

Abstract RU 2731514 C2

FIELD: biotechnology; medicine; agriculture.

SUBSTANCE: invention refers to genetic engineering and can be used in biotechnology, medicine and agriculture to develop gene therapy preparations. Disclosed is a gene-therapeutic DNA vector based on a gene-therapeutic DNA vector VTvaf17, carrying a target gene selected from a group of SHH, CTNNB1, NOG, WNT7A genes, to increase the level of expression of this target gene in the human and animal body. Gene-therapeutic DNA vector VTvaf17-SHH, or VTvaf17-CTNNB1, or VTvaf17-NOG, or VTvaf17-WNT7A, has a nucleotide sequence SEQ ID No. 1, or SEQ ID No. 2, or SEQ ID No. 3, or SEQ ID No. 4, respectively. Invention also discloses a method of producing said vector, use of a vector, an Escherichia coli strain carrying said vector, as well as a method of producing said vector on an industrial scale.

EFFECT: invention can be used for safe application for human and animal genetic therapy.

14 cl, 20 ex, 15 dwg

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RU 2 731 514 C2

Authors

Savelieva Natalia

Dates

2020-09-03Published

2018-12-21Filed