FIELD: biotechnology; medicine; agriculture.
SUBSTANCE: invention refers to genetic engineering and can be used in biotechnology, medicine and agriculture to develop gene therapy preparations. Disclosed is a gene-therapeutic DNA vector based on a gene-therapeutic DNA vector VTvaf17, carrying a target gene selected from a group of SHH, CTNNB1, NOG, WNT7A genes, to increase the level of expression of this target gene in the human and animal body. Gene-therapeutic DNA vector VTvaf17-SHH, or VTvaf17-CTNNB1, or VTvaf17-NOG, or VTvaf17-WNT7A, has a nucleotide sequence SEQ ID No. 1, or SEQ ID No. 2, or SEQ ID No. 3, or SEQ ID No. 4, respectively. Invention also discloses a method of producing said vector, use of a vector, an Escherichia coli strain carrying said vector, as well as a method of producing said vector on an industrial scale.
EFFECT: invention can be used for safe application for human and animal genetic therapy.
14 cl, 20 ex, 15 dwg
