METHOD FOR ISOLATED FRACTION RETROGRADE HEPATIC PERFUSION IN EXPERIMENT Russian patent published in 2021 - IPC A61B17/00 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, particularly to surgery, oncology, transplantology. After the corpse liver explantation, the perfusion is determined; followed by ligating a round hepatic ligament, mobilizing a portal vein, its right and left branches, a hepatic artery, its right and left branches, and a retro-hepatic segment of the inferior vena cava with ligature of short liver veins. That is followed by cholecystectomy. That is followed by successively placing the cannula with an outer diameter of 2–4 mm into a branch of the hepatic artery and a cannula of an external diameter of 6–12 mm into a branch portal vein of the perfused portion using vascular tourniquets. Hepatic veins participating in the venous outflow are squeezed at their bases on the side of the perfused part at the side to exclude leakage of the perfusate in the inferior vena cava. Further, the perfusion contour is formed by connecting the inflow systems from the reservoir with the perfusate to the preparation, to the arterial cannulas, outflow - from the liver to the reservoir - to the venous cannulas, the perfusion pump, the thermostatic bath and connectors for connection of the infusion filters, splitters, extensions, tees for the infusion systems. That is followed by performing an isolated retrograde lavage perfusion of the liver in the following: a perfusion direction - an influx onto a branch of the hepatic artery, an outflow from the portal vein branch of the perfusion fraction. Flow rate is 110–650 ml/min, duration is 20–60 min, perfusate temperature is 37–43 °C. Further, during perfusion, a solution of a water-soluble dye with a total volume of 20–50 ml/min is introduced during perfusion in order to determine the inter-lobe border and to control the vascular isolation of the perfusion to be treated. Upon completion of perfusion, the liver preparation is analysed and utilized.

EFFECT: method enables creating a basis for using transplantation technologies in abdominal "ex vivo" surgery, qualitatively isolating the lobes of the liver in order to perform its retrograde selective perfusion with the required preparation solution, as well as does not require additional search for perfusate outflow path and associated increased volume of surgical intervention and anaesthesia.

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