FIELD: biotechnology.
SUBSTANCE: invention provides a method of sequencing hydroxymethylated cell-free DNA. In certain embodiments, the method involves introducing an affine tag only into molecules of hydroxymethylated DNA in a bcDNA sample, enriching DNA molecules marked with an affine tag, and sequencing of enriched DNA molecules.
EFFECT: non-invasive diagnostics by sequencing of 5-hydroxymethylated cell-free DNA is proposed.
25 cl, 11 tbl, 1 ex, 13 dwg
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