METHOD OF STUDY OF GASTRIC AND INTESTINAL MICROBIOTA DURING SUPPRESSION OF COLONIZATION RESISTANCE OF GASTRIC MUCOSA OF EXPERIMENTAL ANIMALS Russian patent published in 2021 - IPC G09B23/28 A61K31/7036 A61P31/04 C12Q1/06 

FIELD: medicine.

SUBSTANCE: invention relates to medicine, specifically to experimental medicine, medical microbiology and infectiology, and can be used for studying the gastric and intestinal microbiota during suppression of colonization resistance of gastric mucosa of experimental animals. A selective action is performed on the gastric microbiota of the antibacterial preparation gentamicin sulfate containing 40 mg of the antibiotic in 1 ml and diluted with isotonic sodium chloride solution to a concentration of 24 mg in 1 ml, by oral administration to conventional white mice once a day for 5 days at a dosage of 2.4 mg per one mouse. Oral administration of gentamicin is accompanied by simultaneous daily collection of feces for 8 days to determine the representatives of the gastric and intestinal microbiota extracted from bodies of white mice by inoculation of feces on a solid nutritional medium in Petri dishes incubated under microaerophilic conditions at a temperature of 37ºC for 24 hours. The obtained results of feces inoculation are then processed based on the amount of grown bacterial colonies and a judgment is made on the number and loss of normobiota of the gastrointestinal tract.

EFFECT: method allows to establish the time frame for the development of infection in laboratory animals, from adhesion of Helicobacter pylori bacteria and colonization of gastric mucosa to determination of morphologically and histologically distinguishable manifestations of the pathological process, and to study the duration of Helicobacter pylori presence in the gastric tissues based on quantitative determination of bacteria excreted from the body with intestinal contents, by creating artificial microecological and microbiological bio-incompatibility of the “pathogen versus host” type in this biotope in presence of suppression of gastric microbiota and associated suppression of local immune defense of gastric mucosa.

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