VACCINE FOR THE PREVENTION OR TREATMENT OF CORONAVIRUS INFECTION BASED ON A GENETIC CONSTRUCT Russian patent published in 2021 - IPC A61K39/215 A61P31/14 C12Q1/6806 C07K16/10 C12N15/50 

FIELD: molecular biology; biotechnology; medicine.

SUBSTANCE: invention relates to molecular biology, biotechnology, medicine. Described is a polynucleotide for expression in cells of a target organism, encoding a fusion protein comprising fragments of proteins M, S, N, E of coronavirus connected by flexible bridges, characterized by the amino acid sequence SEQ ID NO.:1 or SEQ ID NO.:2. Also described is a polynucleotide for expression in cells of a target organism, encoding a fusion protein comprising fragments of proteins M, S, N, E of coronavirus connected by flexible bridges, characterized by the amino acid sequence SEQ ID NO.:1 or SEQ ID NO.:2, characterized in that which also contains a fragment encoding a heterologous secretory sequence. A genetic construct for the expression of the specified polynucleotide in the cells of the target organism is presented. A vaccine for the prevention or treatment of coronavirus infection in humans or animals is presented, containing the specified genetic construct as an active agent in an effective amount, as well as a physiologically acceptable carrier and a buffer solution. In addition, a method for producing a vaccine for the prevention or treatment of coronavirus infection is described, which consists in the fact that the genetic construct is amplified using PCR, purified and mixed with a physiologically acceptable carrier and a buffer solution.

EFFECT: invention can be used for prevention and treatment of coronavirus infection caused by 2019-nCoV.

19 cl, 3 ex