FIELD: biotechnology.
SUBSTANCE: group of inventions relates to biotechnology. The bacterium Corynebacterium glutamicum, which is a producer of the amino acid with a branched side chain, L-valine, is proposed. The bacterium contains a mutation that represents the replacement of guanine with adenine at the -183 position in the nucleotide sequence before the start codon of the first structural ilvB gene of the ilvBNC operon. A method for obtaining L-valine is also proposed, which provides for the cultivation of this bacterium under suitable conditions until the optimal accumulation of the target product and the isolation of the target amino acid from the culture fluid.
EFFECT: group of inventions makes it possible to increase the yield of L-valine compared to the parent strain.
2 cl, 3 tbl, 6 ex
| Title | Year | Author | Number |
|---|---|---|---|
| L-LYSIN-PRODUCING CORYNEFORMIC BACTERIUM WITH INACTIVE LTBR GENOME AND METHOD FOR OBTAINING OF L-LYSIN USING THIS BACTERIUM | 2016 |
|
RU2639247C1 |
| L-LYSINE-PRODUCING CORYNEFORM BACTERIUM WITH MUTANT GENE FUSA AND METHOD FOR OBTAINING L-LYSINE WITH APPLICATION THEREOF | 2015 |
|
RU2612159C1 |
| SMALL SUBUNIT OF ISOZYME III AND ISOZYME III OF ACETOHYDROXYACID SYNTHETASE FROM ESCHERICHIA COLI, DNA FRAGMENT (VARIANTS), STRAIN OF BACTERIUM ESCHERICHIA COLI AS PRODUCER OF L-VALINE (VARIANTS) AND METHOD FOR PREPARING L-VALINE | 2000 |
|
RU2209246C2 |
| METHOD OF PRODUCING L-LEUCINE, L-VALINE, L-ISOLEUCINE, ALPHA-KETOISOVALERATE, ALPHA-KETO-BETA-METHYLVALERATE OR ALPHA-KETOISOCAPROATE USING RECOMBINANT CORYNEBACTERIUM CONTAINING ILVBN OPERON THAT CAN BE INDUCED BY PROPIONATE | 2014 |
|
RU2675506C2 |
| STRAINS OF BACTERIUM ESCHERICHIA COLI - PRODUCERS OF L-LEUCINE (VARIANTS) | 1997 |
|
RU2140450C1 |
| MUTANT ACETOLACTASE SYNTHASE AND METHOD OF PRODUCING BRANCHED L-AMINO ACIDS | 2006 |
|
RU2355763C2 |
| SELF-INDUCED EXPRESSION SYSTEM AND ITS APPLICATION FOR OBTAINING USEFUL METABOLITES BY MEANS OF BACTERIA OF Enterobacteriaceae FAMILY | 2013 |
|
RU2549708C2 |
| METHOD TO PRODUCE L-AMINO ACID OF GLUTAMATE FAMILY USING CORYNEFORMIC BACTERIUM | 2011 |
|
RU2496867C2 |
| METHOD OF OBTAINING L-AMINO ACID OF GLUTAMATE FAMILY, OR L-BALINE WITH APPLICATION OF BACTERIUM BELONGING TO GENUS Escherichia | 2009 |
|
RU2418064C2 |
| L-THREONINE OR L-ARGININE TECHNIQUE USING BACTERIUM OF Eschrichia GENUS WITH INACTIVATED aldH GENE | 2006 |
|
RU2330882C2 |
