FIELD: biotechnology and medicine.
SUBSTANCE: invention relates to biotechnology and medicine. The invention is a test system and a method for the differentiated (separate) detection of all classes of specific antibodies to the nucleocapsid and the receptor-binding domain of the spike protein of the coronavirus severe acute respiratory syndrome-2 (SARS-CoV-2) in order to assess the immune response to the transferred or current coronavirus COVID-19 infection, laboratory diagnosis of the current infection in combination with other studies, confirmation of positive or questionable test results, minimization of false positive test results. Includes an immunosorbent based on coronavirus antigens, which are the receptor-binding domain (RBD) and nucleoprotein (N) of the SARS-CoV-2 coronavirus, and detecting reagents, the above antigens are sorbed in different wells of plates for enzyme-linked immunosorbent assay, 96-well collapsible polystyrene tablets.
EFFECT: invention allows determining a standardized relative amount of antibodies to different proteins of the virus due to the standard setting conditions and a single level of the threshold value (cutoff), provides increased sensitivity, simplification and ease of analysis, while in combination with other studies can confirm positive or questionable research results, minimizing false positive results (FPR).
3 cl, 8 tbl, 4 ex
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| METHOD FOR ENZYME IMMUNOASSAY DETERMINATION OF THE LEVEL OF ANTIGEN-RECOGNIZING B-LYMPHOCYTE RECEPTORS REPRESENTED BY MEMBRANE-SPECIFIC RBD PROTEIN SARS-COV-2, IgG ANTIBODIES | 2021 |
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| THE STRAIN OF HYBRID CULTURED HOMO SAPIENS/Mus MUSCULUS CELLS Hu-C6D7-RBD IS A PRODUCER OF HUMAN MONOCLONAL ANTIBODIES SPECIFIC TO THE RBD DOMAIN S OF THE SARS-CoV-2 VIRUS PROTEIN | 2022 |
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| METHOD FOR CARRYING OUT AN ENZYME-LINKED IMMUNOSORBENT ASSAY FOR THE DETECTION OF ANTIBODIES IN A HUMAN BIOLOGICAL SAMPLE SPECIFIC TO THE SARS-COV2 HUMAN CORONAVIRUS, A TEST SYSTEM | 2021 |
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