FIELD: biotechnology.
SUBSTANCE: group of inventions relates to biotechnology, namely genetic engineering. Described is a recombinant protein of receptor-binding domain (RBD) – a structural domain of the spike glycoprotein SARS-CoV-2. Disclosed is a method of producing said protein. Recombinant RBD S-protein of the coronavirus SARS-CoV-2 has size of 437 amino acids with a molecular weight of 48.61 kDa. This protein is obtained by transformation of cells of the strain of methylotrophic yeast Pichia pastoris SuperMan 5-23 with a plasmid synthesized in Escherichia coli cells, containing a sequence in accordance with the sequence RBD Cov19: MASNFRAQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLAESNKKFLLFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGHHHHHH, their cultivation at temperature of 30 °C for 24 hours in BMGY medium with composition: 1% yeast extract, 2% peptone, 100 mM potassium phosphate buffer with pH=6.0, 1.34% YNB, 4×10-5% biotin, 1% glycerol and 100 mcg/ml kanamycin and separated by affinity chromatography.
EFFECT: invention can be used in creating ELISA test systems and for designing vaccines against coronavirus infection – COVID-19.
2 cl, 2 dwg, 5 ex
| Title |
Year |
Author |
Number |
| ARTIFICIAL GENETIC CONSTRUCT FOR THE HETEROLOGOUS EXPRESSION OF THE S-PROTEIN RECEPTOR-BINDING DOMAIN IN A FUSED POLYPEPTIDE CHAIN WITH A NUCLEOCAPSID PROTEIN |
2022 |
- Okhrimenko Ivan Stanislavovich
- Zagriadskaia Iuliia Aleksandrovna
- Mishin Aleksei Viktorovich
- Borshchevskii Valentin Ivanovich
- Shevchenko Marina Aleksandrovna
|
RU2801597C1 |
| RECOMBINANT PLASMID PVBL-RBDOMIK PROVIDING SYNTHESIS AND SECRETION OF THE RECOMBINANT RECEPTOR-BINDING DOMAIN (RBD) OF THE SARS-COV-2 CORONAVIRUS LINE B.1.1.529 IN MAMMALIAN CELLS. |
2021 |
- Viktorov Denis Aleksandrovich
- Toropovskii Andrei Nikolaevich
- Shcherbakov Dmitrii Nikolaevich
- Nikitin Aleksei Georgievich
|
RU2772905C1 |
| INTEGRATIVE PLASMID VECTOR pVEAL3-RBDdel, PROVIDING SYNTHESIS AND SECRETION OF RECOMBINANT PROTEIN OF RECEPTOR-BINDING DOMAIN RBDdelta OF CORONAVIRUS SARS-CoV-2 IN MAMMALIAN CELLS, RECOMBINANT CELL LINE STRAIN CHO-K1-RBDdelta AND RECOMBINANT PROTEIN RBDdelta SARS-CoV-2 PRODUCED BY CELL LINE STRAIN |
2023 |
- Nesmeyanova Valentina Sergeevna
- Merkuleva Yuliya Aleksandrovna
- Isaeva Anastasiya Aleksandrovna
- Shcherbakov Dmitrij Nikolaevich
- Volkova Natalya Vyacheslavovna
- Belenkaya Svetlana Valerevna
- Borgoyarkova Mariya Borisovna
- Volosnikova Ekaterina Aleksandrovna
- Esina Tatyana Igorevna
- Zajkovskaya Anna Vladimirovna
- Pyankov Oleg Viktorovich
- Ilichev Aleksandr Alekseevich
|
RU2816175C1 |
| RECOMBINANT PLASMID PVBL-RBDDELTA PROVIDING SYNTHESIS AND SECRETION OF THE RECOMBINANT RECEPTOR-BINDING DOMAIN (RBD) OF THE SARS-COV-2 CORONAVIRUS LINE B.1.617.2 IN MAMMALIAN CELLS |
2021 |
- Toropovskii Andrei Nikolaevich
- Viktorov Denis Aleksandrovich
- Shcherbakov Dmitrii Nikolaevich
- Nikitin Aleksei Georgievich
|
RU2772904C1 |
| POLYPEPTIDE WITH ANTI-SARS-COV-2 ACTIVITY AND PROLONGED CIRCULATION TIME IN BLOOD FLOW |
2023 |
- Bobik Tatyana Vladimirovna
- Simonova Mariya Aleksandrovna
- Rushkevich Natalya Yurevna
- Kostin Nikita Nikolaevich
- Skryabin Georgij Andreevich
- Shulga Aleksej Anatolevich
- Konovalova Elena Valerevna
- Lukyanova Tamara Ivanovna
- Ziganshin Rustam Khusmanovich
- Proshkina Galina Mikhajlovna
- Gabibov Aleksandr Gabibovich
- Deev Sergej Mikhajlovich
|
RU2822355C1 |
| POLYPEPTIDE MODULE FOR BINDING CONSERVATIVE EPITOPE OF RECEPTOR-BINDING DOMAIN OF SPIKE PROTEIN OF SARS-CoV-2 CORONAVIRUS |
2022 |
- Tillib Sergej Vladimirovich
- Vorobev Pavel Olegovich
|
RU2809183C1 |
| ARTIFICIAL GENE CODING A BICISTRONIC STRUCTURE FORMED BY RECEPTOR-BINDING DOMAIN SEQUENCES OF THE GLYCOPROTEIN S OF THE SARS-COV-2 CORONAVIRUS, P2A-PEPTIDE AND GLYCOPROTEIN G VSV, RECOMBINANT PLASMID PSTEM-RVSV-STBL_RBD_SC2, PROVIDING EXPRESSION OF ARTIFICIAL GENE, AND A RECOMBINANT STRAIN OF VESICULAR STOMATITIS VIRUS RVSV-STBL_RBD_SC2, USED TO CREATE A VACCINE AGAINST SARS-COV-2 CORONAVIRUS |
2020 |
- Imatdinov Ilnaz Ramisovich
- Bochkareva Mariya Dmitrievna
- Prudnikova Elena Yurevna
- Tishin Anton Evgenevich
- Pyankov Oleg Viktorovich
- Gavrilova Elena Vasilevna
- Maksyutov Rinat Amirovich
|
RU2733831C1 |
| INTEGRATIVE PLASMID VECTOR PVEAL2-S-RBD, PROVIDING THE EXPRESSION AND SECRETION OF THE RECOMBINANT RECEPTOR-BINDING DOMAIN (RBD) OF THE SARS-COV-2 CORONAVIRUS IN MAMMALIAN CELLS, THE RECOMBINANT CHO-K1-RBD CELL LINE STRAIN AND THE RECOMBINANT SARS-COV-2 RBD PROTEIN PRODUCED BY THE SPECIFIED STRAIN OF THE CELL LINE CHO-K1-RBD |
2021 |
- Merkuleva Yuliya Aleksandrovna
- Shcherbakov Dmitrij Nikolaevich
- Belenkaya Svetlana Valerevna
- Isaeva Anastasiya Aleksandrovna
- Nesmeyanova Valentina Sergeevna
- Shanshin Daniil Vasilevich
- Volkova Natalya Vyacheslavovna
- Ilichev Aleksandr Alekseevich
- Karpenko Larisa Ivanovna
|
RU2752858C1 |
| SINGLE-DOMAIN NANO-ANTIBODIES AGAINST SPIKE PROTEIN OF SARS-CoV-2 VIRUS |
2021 |
- Chumakov Stepan Petrovich
- Zhuchkov Vladislav Aleksandrovich
|
RU2794141C2 |
| ARTIFICIAL GENE STBL_RBD_TRM_SC2, CODING A BICISTRONIC STRUCTURE FORMED BY THE SARS-COV-2 CORONAVIRUS GLYCOPROTEIN S RECEPTOR-BINDING DOMAIN SEQUENCES, TRANSMEMBRANE REGION, P2A-PEPTIDE AND GLYCOPROTEIN G VSV, RECOMBINANT PLASMID PSTEM-RVSV-STBL_RBD_TRM_SC2, PROVIDING EXPRESSION OF ARTIFICIAL GENE, AND A RECOMBINANT STRAIN OF VESICULAR STOMATITIS VIRUS RVSV-STBL_RBD_TRM_SC2, USED TO CREATE A VACCINE AGAINST SARS-COV-2 CORONAVIRUS |
2020 |
- Imatdinov Ilnaz Ramisovich
- Bochkareva Mariya Dmitrievna
- Prudnikova Elena Yurevna
- Tishin Anton Evgenevich
- Pyankov Oleg Viktorovich
- Gavrilova Elena Vasilevna
- Maksyutov Rinat Amirovich
|
RU2733832C1 |
