METHOD FOR CULTIVATING TOXOCARA LARVAE FOR DIAGNOSING TOXOCARIASIS IN CATS Russian patent published in 2021 - IPC A61K39/44 

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of microbiology and pertains to a method for cultivating toxocara larvae for diagnosing toxocariasis in cats. The substance of the method consists in the fact that eggs are embedded in 0.9% saline solution, the preparation is washed with saline solution into a Petri dish, the suspension is subjected to thermostating and centrifugation at a temperature of 39 to 40° for 7 hours with periodic stirring. In the course of evaporation, 0.9% saline solution is added, and after holding in a thermostat, 2% glutamine is added to the suspension in an amount of 1.5 g/2.5 ml. Further, in the process of changing the stages of larvae formation, at the stage of mobile larvae, the dosage of glutamine is increased to 1.9 g. 125 mg of nystatin and 250 mg of amoxicillin are therein added to the suspension at the initial stage of larval development once every 3 to 5 days. The preparations are added at intervals of 45 minutes. Then, in the process of larvae cultivation, after adding nystatin and amoxicillin and further in the course of activation of objects suppressed by nystatin and amoxicillin, the resulting suspension is daily exposed to an infrared lamp for 25 to 35 minutes for 4 weeks until 95% of the specimens are stabilised, followed by settling the suspension containing invasive mobile T.cati larvae in egg shells for 18 hours. To destroy the egg shell, an ultrasonic disintegrator microprobe with a voltage of 20 kHz is placed in the resulting precipitate for 15 minutes, and after the L2 stage larvae of feline toxocara are released from the egg shell structures, the resulting culture is centrifuged for 10 minutes.

EFFECT: invention provides a possibility of cultivating toxocara eggs in a short time with a maximum yield of viable larvae.

3 cl, 1 ex