FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology and represents a method for cultivating larvae of the nematodes of the suborder Ascaridata, carried out as follows: fertilized Ascaris suum eggs are incubated for 24 hours at 27°C in artificial gastric juice with the addition of a chitosan solution at a final concentration of 0.001%. Then the eggs are washed from artificial gastric juice and chitosan, centrifuged for 10 minutes at 1000 rpm and incubated in penicillin vials with saline in a volume of 2 ml for 30 days. After 30 days of incubation, the number of larvae hatched from the eggs is counted.
EFFECT: invention makes it possible to increase the percentage of hatching of larvae from eggs.
1 cl, 2 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR LARVAL NEMATODE ASCARIDATA CULTURE | 2013 |
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