FIELD: medicine; pharmaceutics.
SUBSTANCE: group of inventions relates to a recombinant lactic acid bacterium (LAB) for treating type 1 diabetes (T1D) in a human, a pharmaceutical composition containing said LAB, use of said LAB and composition in treating T1D in humans. Recombinant LAB contains two chromosomally integrated exogenous nucleic acids, wherein the first exogenous nucleic acid codes human interleukin-10 (hIL-10), where said hIL-10 contains a substitution of proline for alanine in position 2 in mature hIL-10, and the second exogenous nucleic acid codes human proinsulin (hPINS). Said ICD constitutively expresses and secretes said hIL-10 and said hPINS, has no activity of trehalose-6-phosphate phosphorylase (TrePP) and has no activity of component IIC of cellobiose-specific phosphotransferase system (PtcC). Said first exogenous nucleic acid is transcriptionally regulated by the hllA (PhllA) promoter and/or integrated into the chromosome in the thyA locus of said recombinant LAB.
EFFECT: inventions provide effective treatment of T1D.
22 cl, 18 dwg, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| COMPOSITIONS AND METHODS FOR TREATING TYPE 1 DIABETES | 2017 |
|
RU2760997C2 |
| MUCOADHESIVE MICROORGANISM | 2017 |
|
RU2762940C2 |
| CHLORIDE-INDUCED PROKARYOTIC EXPRESSION SYSTEM | 2019 |
|
RU2812766C2 |
| VECTOR, CO-EXPRESSING MOLECULES FOR VACCINATION AND COSTIMULATORY MOLECULES | 2016 |
|
RU2714157C2 |
| OPTIONS, COMPOSITIONS, AND METHODS FOR USE OF ENDONUCLEASE CBLB | 2018 |
|
RU2779097C2 |
| COMPOSITIONS AND METHODS FOR BIOLOGICAL PRODUCTION OF LACTATE FROM C1-COMPOUNDS USING TRANSFORMANTS OF LACTATE DEHYDROGENASE | 2014 |
|
RU2710714C2 |
| MUC16-SPECIFIC CHIMERIC ANTIGEN RECEPTORS AND THEIR USE | 2019 |
|
RU2795198C2 |
| METHODS FOR TREATING CANCER, INCLUDING TIGIT-BINDING AGENTS | 2017 |
|
RU2765410C2 |
| DELIVERY OF THERAPEUTIC POLYPEPTIDES VIA PSEUDOTYPED ONCOLYTIC VIRUSES | 2017 |
|
RU2758007C2 |
| OPTIONS, COMPOSITIONS, AND METHODS FOR USE OF HOMING-ENDONUCLEASE PD-1 | 2017 |
|
RU2781083C2 |
