NEW PROBIOTIC BASED ON A CONSORTIUM OF SPORE-FORMING BACTERIA FOR AQUACULTURE AND ANIMALS AND A METHOD OF ITS PRODUCTION Russian patent published in 2023 - IPC A61K35/742 C12N1/20 C12R1/07 

FIELD: microbiology; biotechnology.

SUBSTANCE: invention relates to a probiotic for aquaculture. Probiotic for aquaculture, namely for brine shrimp and brine shrimp cysts which contains freeze-dried biomass of Bacillus toyonensis VKPM B-13249 and Bacillus pumilus VKPM B-13250 strains and maltodextrin filler in the following ratio, wt. %: Bacillus toyonensis — 5, Bacillus pumilus — 15, maltodextrin — 80, the number of viable bacteria is at least 1×10¹⁰ CFU/g. A method of producing a probiotic preparation for aquaculture, namely for cysts of Artemia crustacean and shrimp, including separate cultivation of strains of Bacillus toyonensis VKPM B-13249 and Bacillus pumilus VKPM B-13250 on molasses-corn medium in fermentation plants at pH 6.8–7.0 and temperature 37°C, air flow 100–150 l/min, mixing speed 250–500 rpm; pO₂ — 30–50% in the phase of intensive growth of culture, the duration of fermentation 18–24 h until the concentration of cells in the spore form of 93–95%; concentration of the culture fluid in a flow centrifuge at a speed of 15,000 rpm with a culture fluid feed rate of 100–120 l/h; mixing concentrates of strains with cryoprotective medium; freezing and freeze drying; thorough mixing for 30–60 minutes of the resulting dry biomass of bacteria in a ratio of 1:3, followed by the addition of maltodextrin filler to 100% of the mass of the product.

EFFECT: group of inventions described above provides for obtaining a product that has a high titer, antagonistic activity against pathogenic, opportunistic microorganisms, a long shelf life and efficiency in the incubation of Artemia crustacean cysts and Rosenberg shrimp.

3 cl, 2 dwg, 3 tbl, 4 ex