FIELD: medicine.
SUBSTANCE: group of inventions relates to the determination of activity of a therapeutic cell composition. A method is disclosed for the determination of activity of a sample containing auxiliary cells (hereinafter – FC) and/or alpha-beta TCR+ T-cells, including bringing the sample containing FC and/or alpha-beta TCR+ T-cells into contact with mitogenic stimulus; and determination of a number of FC and/or alpha-beta TCR+ T-cells in the sample, in which ribosome protein S6 is phosphorylated (hereinafter – pS6). The number of FC and/or alpha-beta TCR+ T-cells in the sample, in which pS6 is phosphorylated, characterizes the activity of FC and/or alpha-beta TCR+ T-cells, respectively, in the sample. The method is carried out before freezing FC and/or alpha-beta TCR+ T-cells, or is carried out on FC and/or alpha-beta TCR+ T-cells, which were frozen. A method for the determination of a therapeutic dose of FC and/or alpha-beta TCR+ T-cells, using the specified method for the determination of activity, and a product for implementation of the specified method for the determination of activity are also disclosed.
EFFECT: group of inventions provides for a fast and quantitative method for the assessment of activity of a therapeutic cell composition.
15 cl, 5 dwg, 12 tbl, 12 ex
| Title |
Year |
Author |
Number |
| METHODS AND COMPOSITIONS FOR OBTAINING GENETICALLY ENGINEERED CELLS |
2018 |
|
RU2795454C2 |
| RAPID ASSAYS FOR T-CELL ACTIVATION BY RNA MEASUREMENTS USING FLOW CYTOMETRY |
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- Bushkin Yurij
- Dzhennaro Mariya Laura
- Tiyagi Sandzhaj
- Pajn Richard
|
RU2671707C2 |
| IMPROVED COMPOSITIONS BASED ON T-CELLS |
2015 |
|
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| IMMUNE MODULATORS, PREPARATIONS AND COMPOSITIONS CONTAINING IMMUNE MODULATORS, ACTIVITY TESTS OF IMMUNE MODULATORS, PREPARATIONS AND COMPOSITIONS CONTAINING THEM, AND METHODS |
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- Lisonbi Dehvid
- Makkosland Kalvin U
- Bennett Richard Kh
- Vogan Brent M
- Lefler Shejn M
|
RU2518341C2 |
| IMMUNE MODULATORS, PREPARATIONS AND COMPOSITIONS CONTAINING IMMUNE MODULATORS, ACTIVITY TESTS OF IMMUNE MODULATORS, PREPARATIONS AND COMPOSITIONS CONTAINING THEM, AND METHODS |
2014 |
- Lisonbi Devid
- Makkosland Kalvin U.
- Bennett Richard Kh.
- Vogan Brent M.
- Lefler Shejn M.
|
RU2619555C2 |
| METHOD AND COMPOSITIONS FOR IMMUNOMODULATION |
2015 |
- Mata-Fink Dzhordi
- Raund Dzhon
- Afeyan Nubar B.
- Kakhvedzhan Avak
|
RU2736495C2 |
| COMPOSITIONS AND METHOD FOR IMMUNOSUPPRESSION |
2001 |
- Strom Terri B.
- Maslinski Vlodzimezh
- Zkheng Ksin Ksiao
- Kim Jon Su
- Lakra Sil'Vi Ferrari
|
RU2270691C2 |
| METHODS OF EXPANSION OF ANTIGEN-SPECIFIC CAR-T-CELLS, COMPOSITIONS AND THEIR USE |
2019 |
- Aftab, Blake, T.
- Pham, Christina
- Shen, Rhine
- Wu, Michelle
|
RU2800920C2 |
| SPECIFIC BINDING MOLECULES |
2020 |
- Konnoli, Meri Margerit
- Torreno, Sara Krespijo
- Sakling, Richard
- Dembek, Marsin
- Donoso, Khose
- Viderkhold, Katrin
- Noks, Endryu
|
RU2825837C2 |
| METHODS AND COMPOSITIONS FOR DIAGNOSTICS AND TREATMENT OF AUTOIMMUNE DISEASE, RESULTING FROM MULTIPLE SCLEROSIS |
2009 |
- Koulz Alazdehr Dzh.
- Dzhounz Dzhoann L.
- Kompston Alastehr
|
RU2563521C2 |
