FIELD: immunology.
SUBSTANCE: disclosed is a method of producing T cells, according to which the stages of activation, stimulation, transduction with the help of a viral vector containing a polynucleotide, coding a T-cell receptor (TCR) or a chimeric antigenic receptor (CAR), as well as culturing transduced T-cells for proliferation are performed in the presence of a phosphatidyl-inositol-3-kinase (PI3K) inhibitor.
EFFECT: use of said inhibitor increases CD62L expression; thus obtained T-cells can be used in adoptive therapy.
21 cl, 6 dwg, 6 ex
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