FIELD: biotechnology.
SUBSTANCE: inventions group relates to biotechnology. A complex biological preparation for optimizing the mineral nutrition of plants, protecting against phytopathogens, increasing productivity and a method for its production, including the joint cultivation of bacterial strains Agrobacterium radiobacter 204, Lelliottia nimipressuralis CCM 32-3 and Paenibacillus polymyxa P13NK, are proposed, while the corn-molasses nutrient medium is seeded with mother cultures strains A. radiobacter 204 and L. nimipressuralis CCM 32-3 simultaneously in an amount of 1.5% of each strain of the volume of the medium, after which the mother culture of the strain P. polymyxa P13NK (2% of the culture volume) is introduced into the 24-hour culture of two strains ) and cultured for 48 hours until a titer of 109 CFU per 1 ml is obtained at 28–30°C at a shaker rotation speed of 170–220 rpm. The titer of Agrobacterium radiobacter 204 in the biological product is 48%, Lelliottia nimipressuralis CCM 32-3 - 49% and Paenibacillus polymyxa P13NK - 3% of the total CFU.
EFFECT: inventions provide increase of seed germination, improvement of sowing properties and grain productivity of agricultural crops.
2 cl, 2 dwg, 10 tbl, 9 ex
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RU2760743C1 |
PHOSPHATE-MOBILIZING STRAIN OF SOIL BACTERIA LELLIOTTIA NIMIPRESSURALIS CCM 32-3 AND A BIOLOGICAL PREPARATION BASED ON IT TO OPTIMIZE THE MINERAL NUTRITION OF PLANTS, STIMULATE THEIR GROWTH AND INCREASE YIELDS | 2018 |
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RU2752903C1 |
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RU2800257C1 |
POLYFUNCTIONAL BIOPREPARATION BASED ON STRAIN OF RHIZOBACTERIA PAENIBACILLUS POLYMYXA WITH SYSTEM OF USEFUL PROPERTIES FOR PROTECTING PLANTS FROM PHYTOPATHOGENS, INCREASING CROP YIELD, IMPROVING SOWING PROPERTIES OF SEEDS AND STIMULATING BIOLOGICAL ACTIVITY OF RHIZOSPHERE | 2024 |
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RU2838661C1 |
FLAVOBACTERIUM SP_ 72 ASSOCIATIVE BACTERIA STRAIN, ACTIVE NITROGEN FIXATOR AND PRODUCER OF PHYSIOLOGICALLY ACTIVE SUBSTANCES USED TO INCREASE RICE YIELD | 2019 |
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RU2728641C1 |
TECHNOLOGY FOR PRODUCTION OF LIQUID FORM OF COMBINED BIOLOGICAL PRODUCT BASED ON BACILLUS SUBTILIS AND BACILLUS MEGATERIUM VAR. PHOSPHATICUM | 2020 |
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RU2761117C1 |
METHOD FOR PREPARING BIOHUMUS-BASE COMBINED FERTILIZING SOIL | 2004 |
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Authors
Dates
2022-08-01—Published
2022-01-13—Filed