FIELD: nucleotide sets manufacturing.
SUBSTANCE: present invention relates to a method for manufacturing sets of nucleotide and/or other molecular sequences using microparticles bearing labels that are one-to-one associated with manufactured sequences, characterized in that the set of microparticles is manufactured in a single synthetic procedure that does not limit the number of microparticles, the number of labels, the length and the composition of the sequences, oligonucleotide or otherwise; where the synthesis is carried out cyclically over the entire set of microparticles, divided into a small number of subgroups in each cycle, for which the sorter sorts the microparticles according to a label unique for each synthesized sequence in each cycle of synthesis and adds a nucleotide or other sequence element to the sequences on each subgroup sorted by labels particles, in each cycle of synthesis; the synthesis algorithm and the corresponding algorithm of the software that controls the sorters include the following sequence of actions: a) formation of a correspondence base between labels on microparticles and molecular sequences synthesized on these particles; b) the formation of several sets of labels at each step of the synthesis, according to the number of molecular elements introduced in sequence in each cycle of synthesis; in particular, for the synthesis of oligonucleotide sequences - four sets, according to the number of nucleotides; c) sorting microparticles into subgroups corresponding to the selected sets of labels for each step of the synthesis; d) adding the desired nucleotide, or another element of the sequence, to the already immobilized parts of the synthesized sequences immobilized on the microparticles of each subgroup corresponding to the set of labels selected for a given operation in a given synthesis cycle; the step of adding an element includes a degenerate case of adding an "empty" element of the sequence, when one of the subgroups is not modified; e) mixing subgroups of particles and proceeding to sorting for the next cycle of synthesis.
EFFECT: present invention provides a set of combinatorially or otherwise labeled microparticles, each of which carries user-specified molecular sequences for each label, such as oligonucleotides and their derivatives.
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