FIELD: biotechnology.
SUBSTANCE: system and a method for analyzing a phenotype and a sequence of polynucleotides of a biological particle are proposed. The system contains an analyzer of a phenotype of a biological particle, a generator of an identifiable oligonucleotide coding carrier, a microreactor, a sequencer of a pre-determined sequence of an encoding oligonucleotide and a sequence of polynucleotides of a biological particle. The method includes optical analysis of a biological particle to determine the phenotype, recording the phenotype, generating a carrier of coding oligonucleotides, placing a biological particle and the carrier in the microreactor, lysis of a biological particle, ligation of coding oligonucleotides with polynucleotides of a biological particle, sequencing to determine the sequence of polynucleotides of a biological particle and comparing the phenotype of a biological particle with the sequence of polynucleotides of a biological particle.
EFFECT: inventions provide an accurate determination of genome/transcriptome and phenotype for each biological particle.
15 cl, 24 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR MANUFACTURING SETS OF NUCLEOTIDE AND/OR OTHER MOLECULAR SEQUENCES USING MICROPARTICLES BEARING LABELS UNIVERSALLY ASSOCIATED WITH MANUFACTURED SEQUENCES | 2022 |
|
RU2784192C1 |
| HIGH THROUGHPUT SEQUENCING OF POLYNUCLEOTIDE LIBRARIES AND TRANSCRIPTOME ANALYSIS | 2018 |
|
RU2790291C2 |
| AFFINITY CONJUGATES - OLIGONUCLEOTIDE AND THEIR APPLICATIONS | 2016 |
|
RU2778754C2 |
| HIGH-PERFORMANCE SINGLE CELL SEQUENCING WITH REDUCED AMPLIFICATION ERROR | 2019 |
|
RU2744175C1 |
| RAD3 POLYPEPTIDE ATR HOMOLOGUES, POLYNUCLEUTIDES AND RELATED METHODS AND MATERIALS | 1996 |
|
RU2252256C2 |
| METHOD AND COMPOSITION FOR ANALYZING CELLULAR COMPONENTS | 2016 |
|
RU2717491C2 |
| PROTEIN IFNAB-BPII ELICITING ABILITY TO BIND INTERFERON-α/β, ITS PRECURSOR AND FUSION PROTEINS, METHOD FOR PREPARING IFNAB-BPII, DNA MOLECULES, EXPRESSING VECTOR, PHARMACEUTICAL COMPOSITION | 1995 |
|
RU2232811C2 |
| PHAGE φ-mru POLYNUCLEOTIDES AND POLYPEPTIDES AND THEIR APPLICATION | 2008 |
|
RU2520738C2 |
| METHODS FOR DISCRETE AMPLIFICATION OF COMPLETE GENOME | 2016 |
|
RU2736351C2 |
| AFFINE MOLECULE-OLIGONUCLEOTIDE CONJUGATES AND THEIR APPLICATIONS | 2017 |
|
RU2763554C2 |
