METHOD OF DETERMINING NUCLEIC ACID SEQUENCE (VERSIONS) AND KIT FOR USE IN DETERMINATION OF NUCLEIC ACID SEQUENCE Russian patent published in 1999 - IPC

Abstract RU 2143004 C1

FIELD: genetic engineering. SUBSTANCE: invention provides methods and kit for rapid and high- efficiency creation of nucleic acid sequence by way of hybridization of two series of small specimens of oligonucleotides with known sequences. Extremely great nucleic acid molecules, including chromosomes and undissolved RNAs, can be arranged into sequences with no preliminary cloning and subcloning. Proposed methods can also solve various problems related to technology of creating sequences such as low signal- noise ratio and difficulty caused by selective attachment of many nucleic acid fragments to surface, preparation of many longer and more complex specimens, and applying labels on larger number of substances. EFFECT: facilitated determination procedure. 41 cl, 10 dwg, 9 ex

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RU 2 143 004 C1

Authors

Drmanach Radoe

Dates

1999-12-20Published

1994-09-27Filed