METHOD FOR OBTAINING LIPOSOMAL NANOCONTAINERS WITH IMMOBILIZED ENZYME Russian patent published in 2022 - IPC A61K9/127 A61K38/43 A61K47/24 B82B3/00 

FIELD: chemistry and pharmaceuticals.

SUBSTANCE: invention relates to the field of chemistry and pharmaceuticals, and in particular to a method for obtaining liposomal nanocontainers with an immobilized enzyme, including the preparation of a 0.3% solution of lecithin in ethanol, which, before being placed in a rotary evaporator, is kept for 15 minutes in an ultrasonic bath heated to 55°C and at 60 Hz and then filtered, evaporation of the filtrate in a rotary evaporator, which is carried out using a diaphragm pump with a rotation speed of 2500 revolutions at a water bath temperature of 60°C until a yellow-brown lipid film forms on the flask, adding sodium phosphate buffer with pH 7,4 in a volume equal to the volume of a solution of lecithin in ethanol, and the enzyme preparation is introduced as a solution in sodium phosphate buffer with pH 7,4 and continued to stir for 2 minutes without using vacuum and heating; then placed in an ultrasonic bath without heating for 5 minutes at 60 Hz.

EFFECT: obtaining multilamellar liposomal nanocontainers with an immobilized enzyme, providing targeted delivery of the enzyme preparation by releasing the enzyme in the duodenum.

1 cl, 4 tbl, 1 ex