FIELD: medicine.
SUBSTANCE: methods for digitizing biological preparations using automated microscopic systems and neural networks, used to detect and analyze formed elements in a bone marrow preparation. A method for digitizing a biological bone marrow preparation is proposed, which includes obtaining a smear image of the preparation at a low magnification, introducing the resulting image into the first neural network to obtain bone marrow smear masks, determining the location of areas of interest, transferring areas of interest to the second neural network, and determining the degree of suitability of areas of interest for preparation analysis. Digitization of the preparation is carried out in two stages, at the first stage, the bone marrow preparation, prepared by the slapping method, is placed on the stage of the microscopic system and digitized to obtain digital images at a low magnification of 10x, followed by cross-linking into one image, then the resulting image is entered into the first neural network, which is generative-adversarial with the ability to determine the coordinates of a smear on a slide, build a smear mask and a mask of the central smear spot, determine the coordinates of suitable areas of interest and store them in the computer's memory. Next, the saved areas of interest are introduced into the second neural network to determine the degree of suitability of the visual fields for analysis, while the pretrained second neural network is a deep convolutional neural network based on VGG16 with the ability to sort the found regions of interest according to the degree of suitability for further digitization at high magnification. If the first neural network does not determine the boundaries of the central spot, the mask of the central spot is formed from the smear mask by erosion, the coordinates of suitable areas of interest are determined and stored in the computer memory. Then, the saved areas of interest obtained at a magnification of 10x are divided into fields of view for obtaining images at a magnification of 100x, the selected fields of view are digitized and stored in the computer's memory, while the fields of view are digitized by moving the stage of microscope with the preparation in such a way that the field of view of the camera of the microscopic system was in the upper left corner of a certain area of interest, then an image of this field of view is obtained and transferred to an adjacent field of view, moving from left to right in an odd row and from right to left in an even row until all fields of view covering the area of interest are digitized. The degree of suitability is determined depending on the uniformity of the distribution of cells in the image and the presence or absence of artifacts, such as voids, paint bubbles, scratches.
EFFECT: ensuring high quality of the result of digitization of bone marrow preparations obtained by the slapping method.
2 cl, 8 dwg
