SEPARATION OF ANTIBODIES WITH THREE LIGHT CHAINS USING CATION EXCHANGE CHROMATOGRAPHY Russian patent published in 2023 - IPC A61K39/395 B01D15/36 

FIELD: chemistry.

SUBSTANCE: group of inventions relates to a method for separating H2L3 antibodies from an antibody composition containing H2L3 antibodies and H2L2 antibodies, and this method includes: (i) applying the antibody composition to a cation exchange resin so that the H2L3 antibodies and H2L2 antibodies bind to the resin, and the composition of antibodies has a pH of less than 6.0; (ii) applying the composition for elution with a salt concentration from 300 mm to 600 mm on a cation exchange resin; and (iii) collecting a composition containing H2L2 antibodies eluted from the resin, the said composition containing H2L2 antibodies containing one or more eluted column volumes selected from column volumes 1–9, as well as a method for separating anti-CD123 H2L3 antibodies from an anti-CD123 antibody composition comprising an anti-CD123 antibody H2L3 and an anti-CD123 antibody H2L2, wherein the anti-CD123 antibody H2L3 and anti-CD123 antibody H2L2 comprise the following: (i) heavy chain variable region CDR1, CDR2 and CDR3 sequences of SEQ ID NO: 5–7, respectively, and the sequences of CDR1, CDR2, and CDR3 of the light chain variable region of SEQ ID NO: 8–10, respectively, and (ii) an IgG heavy chain constant domain.

EFFECT: separation of H2L3 antibodies from compositions containing H2L3 antibodies and H2L2 antibodies using a cation exchange resin.

47 cl, 8 dwg, 4 tbl, 3 ex