TECHNETIUM-99m COMPLEX WITH RECOMBINANT ADDRESSED PROTEIN MOLECULES WITH ANKYRIN REPEATS FOR RADIONUCLIDE DIAGNOSTICS OF MALIGNANT LANDSCAPE WITH HER2/NEU OVEREXPRESSION AND METHOD OF ITS OBTAINING Russian patent published in 2024 - IPC A61K51/08 A61K103/10 C07K14/435 

FIELD: medicine; oncology; pharmaceutical chemistry and pharmacology; radiopharmaceutical drugs.

SUBSTANCE: following is proposed: a technetium-99m complex with recombinant targeted protein molecules with ankyrin repeats for radionuclide diagnostics of malignant tumors with overexpression of HER2/neu, designated by the formula [ ^99mTc]Tc-G3-(G₃S)₃C and representing an amino acid sequence

DLGKKLLEAARAGQDDEVRILMANGADV

NAKDEYGLPLYLATAHGHLEIVEVLLNGA

DVNAVDAIGFTPLHLAAFIGHLEIAEVLLK

HGADVNAQDKFGKTAFDISIGNGNEDLAE

ILQKLNGGGSGGGSG-Х,

where Х is ,

where Tc represents ^99mTc. A method of obtaining the specified complex is also proposed, the said method includes the following: a solution is added to a lyophilized mixture of tin (II) chloride dihydrate, sodium salt of gluconic acid and sodium salt of ethylenediaminetetraacetic acid in a mass ratio of (0.5–1.25):67:1.3 sodium pertechnetate, ^99mTc with an activity of 300–1,000 MBq and stirred, then mixed with 500–4,000 μg of protein solution G3-(G ₃ S) ₃ C, incubated at 40–60°C for 20–40 minutes, sterile 0.9% sodium chloride solution is added and the mixture is filtered through a sterilizing filter.

EFFECT: expansion of the arsenal of means for diagnosing malignant tumors with overexpression of HER2/neu.

2 cl, 3 dwg, 1 tbl