METHOD OF PRODUCING POLYMER MICROSPHERES IN MICROFLOW CONDITIONS Russian patent published in 2024 - IPC C08F2/18 C08F212/08 B01J13/16 

FIELD: high-molecular compounds.

SUBSTANCE: invention relates to a method of producing polymer microspheres in microflow conditions, which can be used in a latex agglutination reaction, as well as a base for test systems for multiplex immunoassay. Invention is carried out by a method of producing polymer microspheres in microflow conditions, characterized by that two reagent solutions are pre-prepared, one of which consists of acrylic or methacrylic acid, an initiator, the second solution consists of two components, one of which is water, after which, at temperature of 75–90 °C, using two pumps simultaneously dispensing the first and second reagent solutions into a flow reactor, which is a T-shaped mixer, wherein the first solution, called the dispersed phase, consists of styrene with concentration of 65–66.2 wt.%, acrylic or methacrylic acid in amount of 2.3–5.5 wt.% in terms of styrene, initiator in amount of 2 wt.% in terms of monomers, the rest is cyclohexane, and the second solution, called a continuous phase, consists of 1 wt.% of polyvinyl alcohol, the rest is water, after which continuous and disperse phases are fed into a flow reactor in the form of a T-shaped mixer with a channel diameter at intersection of 100 mcm, at ratio of constant flow rates from 75 to 200 for generation of emulsion drops, then the obtained emulsion from the T-shaped mixer is fed into a coil with an inner diameter of 500–800 mcm, and said reagents are subjected to suspension copolymerisation while heating to 75–90 °C, wherein the initiator used is azobis-isobutyric acid dinitrile.

EFFECT: providing a method of producing a stable suspension of narrowly dispersed polymer microspheres with diameter of 3.7–19.5 mcm and a narrow particle size distribution (coefficient of variation CV 2.2–7.5%), concentration of carboxyl groups on surface is 0.0965–0.2918 mmol/g of microspheres.

1 cl, 8 ex