METHOD FOR DETERMINATION OF ERYTHROCYTE ELASTICITY IN HYPERGLYCEMIA IN WHOLE BLOOD Russian patent published in 2024 - IPC G01N33/49 G01N29/24 

FIELD: medical equipment.

SUBSTANCE: invention relates to a method for determining erythrocyte elasticity in hyperglycemia in whole blood. In the method, whole blood is taken from a patient into a test tube with an anticoagulant, a first portion of the whole blood is centrifuged to obtain blood plasma, part of whole blood is taken from the second part of whole blood for counting the number of erythrocytes in Goryaev chamber, distilled water is poured into two acoustic cells for calibration, the temperature is set to 15–40 °C. Ultrasound in range of 5–15 MHz is supplied to two acoustic cells. Central frequencies of all resonance peaks are recorded in the computer memory in the frequency range of 5–15 MHz, computer calculates average difference of frequency distance between resonance peaks and number of selected resonance peak by formula

where j is serial number of selected resonance peak; N is the number of frequency distances between resonance peaks in the frequency range of 5–15 MHz; f_j is the resonance frequency of the j-th resonance peak; f_j+1 is resonance frequency j+1 of resonance peak, then distilled water is removed from the acoustic cells and whole blood is poured into the first acoustic cell after mixing, and blood plasma is poured into the second acoustic cell. Ultrasound in range of 5–15 MHz is supplied to the acoustic cells; central frequencies of all resonance peaks in the frequency range of 5–15 MHz are recorded in the computer memory for whole blood and blood plasma. Then the value of central frequencies of resonance peaks of the same number is selected for distilled water, blood plasma and whole blood and calculating ultrasound velocities of distilled water, blood plasma and whole blood by formulas

where V^(H2O)1,2 is ultrasound speed in distilled water in acoustic cells; f_j^(H2O)1,2 are center frequencies of resonance peaks of number j for distilled water in acoustic cells; Z_j(H2O)1,2-longitudinal wave numbers in acoustic cells with distilled water; R is the radius of the acoustic cells,

where V^(S)1,2 is the ultrasound velocity in the whole blood in the first acoustic cell and in the blood plasma in the second acoustic cell; f_j^(S)1,2 are center frequencies of resonance peaks of number j for whole blood in the first acoustic cell and in blood plasma in the second acoustic cell; Z_j(S)1,2 are longitudinal wave numbers in whole blood in the first acoustic cell and in blood plasma in the second acoustic cell; R is the radius of the acoustic cells, based on the obtained ultrasound velocities in whole blood in the first acoustic cell and in the blood plasma in the second acoustic cell, the whole blood acoustic parameter (whole blood AcP) is calculated by the whole blood AcP formula = (V whole blood − V_H2O) * 1,000/V_H2O, where V whole blood is the ultrasound speed in whole blood in the first acoustic cell, V_H2O is the ultrasound speed in distilled water in the first acoustic cell; and calculating the blood plasma acoustic parameter (AcP of blood plasma) by the formula AcP of blood plasma = (V blood plasma − V_H2O) * 1,000/V_H2O, where V of blood plasma is the ultrasound velocity in blood plasma in the second acoustic cell; V_H2O is the ultrasound velocity in distilled water in the second acoustic cell, based on the calculated AcP of whole blood, AcP of blood plasma and the number of erythrocytes in whole blood obtained in the Goryaev chamber; erythrocyte elasticity is calculated by formula G = (AcP of whole blood— AcP of blood plasma)/RBC, where RBC is the number of erythrocytes in whole blood.

EFFECT: creation of a more accurate and informative method for determination of erythrocyte elasticity in whole blood in hyperglycemia.

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