METHOD FOR DIAGNOSTICS OF TUMOR-ORIGINATED DISEASES OF INTERNAL ORGANS AND METHOD FOR PREPARING CONTROL SAMPLE FOR CARRYING SAID DIAGNOSTICS METHOD INTO EFFECT (ALTERNATIVES) Russian patent published in 1997 - IPC

FIELD: medicine; laboratory diagnostics. SUBSTANCE: method is based upon analysis of patient's blood sample as compared with control sample. For this purpose, according to first alternative embodiment, variable-frequency ultrasound 3-20 MHz is passed through distilled water (DH₂O) and control sample at 37±0.05 C; through (DH₂O), control sample and blood serum sample at temperatures T₁ and T₂(T₂>T₁) comprised within range 15-40 C (third alternative). Later relative ultrasound velocities Φ₁< 15,2•10^-13 are measured and fact of disease is diagnosed (first alternative). Values Φ₁, and Φ₂ (for blood serum sample) V and relationship Φ₁•Φ₂<270•10^-3 make it possible to diagnose disease (second alternative). Values Φ₁, Φ₂ for temperature T₁ and values Φ₃, Φ₄ for temperature T₂ make it possible to determine value and if П ≅ 300 fact of disease is diagnosed (third alternative). If П value ranges from 301 to 399, additionally is calculated and at П₁16.0 fact of disease is diagnosed; П₂ value is calculated and at П₂<83 fact of disease is diagnosed; П₃ value П₃=((mS)_o•S_o•δ(mS))•10¹¹ is calculated and at П₃<59 fact of disease is diagnosed. Control samples for carrying out method (according to first and second alternatives) are prepared by mixing together 30%-trichloroacetic acid diluted to concentration capable to cause relative ultrasound velocity change (-18,0±0,05)•10^-3 in relation to distilled water, and blood serum sample in volume ratio 1:4, followed by adding to this mixture NaHCO₃ solution taken volume equal to blood serum volume and having relative ultrasound velocity change _{(22,0±0,05)•10} -3 in relation to distilled water. Mixture thus-obtained is subjected to centrifuging at speed 6000 rpm for 1.5 min and supernatant liquid thus-produced is used as control sample. As regards third alternative embodiment of proposed diagnostics method, control sample is obtained by mixing together blood serum sample and trichloroacetic acid, followed by centrifuging mixture at speed 5000 rpm for 2 min. Next NaHCO₃ solution capable to cause relative ultrasound velocity change _{(16,0±0,05)•10} -3 in relation to distilled water is added, and mixture thus-obtained is centrifuged at 5000 rpm for 2 min. Supernatant liquid thus-obtained is used as control sample. EFFECT: greater accuracy of diagnostics due to minimized hyperdiagnostics; expanded range of internal organs that can be diagnosed (atomach, pancreas, lungs, etc). 8 cl