VIRUS-ENCODED REGULATED AGENTS BINDING T-CELLS (CATVERT) OR NK-CELLS (CATVERN) TARGETING CANCER Russian patent published in 2024 - IPC C07K16/28 C12N15/86 C12N15/864 C12N15/113 

FIELD: biotechnology.

SUBSTANCE: disclosed is an olynucleotide for regulating genes, comprising: (a) a first polynucleotide sequence comprising a first portion of an open reading frame encoding a first polypeptide; (b) a second polynucleotide sequence comprising a second portion of an open reading frame encoding a first polypeptide; (c) a third polynucleotide sequence encoding a second polypeptide; and (d) a polynucleotide gene regulation sequence located between the first polynucleotide and the second polynucleotide. Described also is an expression vector containing said polynucleotide. Described is a pharmaceutical composition containing a polynucleotide or a vector and a carrier, optionally a pharmaceutically acceptable carrier. Described is a method of treating cancer in a subject in need thereof, involving administering to the subject an effective amount of a recombinant polynucleotide or a vector or a pharmaceutical composition wherein the polynucleotide or vector expresses a therapeutic anti-tumour antibody or antigen-binding fragment thereof. Disclosed is a method of producing a bispecific antibody or a trispecific antibody in a cell, involving contact of a cell containing a polynucleotide or a vector with an effective amount of an antisense oligonucleotide. Disclosed is a gene regulation kit containing a polynucleotide, or a vector, or a pharmaceutical composition.

EFFECT: invention extends the range of agents for expressing a therapeutic gene for a disease or condition.

55 cl, 49 dwg, 3 ex