EXPRESSION OF ANTIGEN-BINDING PROTEINS IN NERVOUS SYSTEM Russian patent published in 2024 - IPC A61K48/00 C12N15/86 

FIELD: medical biotechnology.

SUBSTANCE: disclosed is a method of expressing a homodimeric bivalent binding element in a cell of the nervous system. Method comprises introducing into a cell an expression cassette for expressing a polypeptide comprising an antibody heavy chain variable domain (V_H), an antibody light chain variable domain (V_L) and an IgG Fc region, where V_H and V_L form an antigen-binding site that specifically binds to the target protein, and wherein said IgG Fc region comprises a single immunoglobulin (Ig) heavy chain portion starting in the hinge region immediately before the papain cleavage site and ending at the C-terminus of the Ig heavy chain, and when expressed in a cell, the two polypeptide molecules form a disulphide-linked homodimeric bivalent binding element specific to the target protein. What is presented is using the expression construct for producing a drug for treating a human patient in need thereof.

EFFECT: method enables to modify cells of the nervous system for expression of binding elements in the brain of patients suffering a neurological disease, such as a neurodegenerative disease.

16 cl, 14 dwg, 5 ex