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IPC

C12Q1/00(2006-01-01)

C12Q1/68(2006-01-01)

C12Q1/6876(2018-01-01)

(21) (22)

Application

2024118652, 2024-07-04

(24)

Start date

2024-07-04

(22)

Actual filing date

2024-07-04

(45)

Published

2024-12-02

(72)

Inventor

Azarova Iuliia EduardovnaSemikina Elena ViktorovnaKlesova Elena IurevnaPolonikov Aleksei Valerevich

(73)

Holder

Federalnoe Gosudarstvennoe Biudzhetnoe Obrazovatelnoe Uchrezhdenie Vysshego Obrazovaniia Gosudarstvennyi Meditsinskii Universitet" Ministerstva Zdravookhraneniia Rossiiskoi Federatsii

METHOD FOR GENOTYPING SINGLE-NUCLEOTIDE VARIANT rs6443265 (T>C) OF HUMAN OGG1 GENE BY REAL-TIME POLYMERASE CHAIN REACTION Russian patent published in 2024 - IPC C12Q1/00 C12Q1/68 C12Q1/6876

Abstract RU 2831204 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, molecular genetic diagnostics. Method for genotyping a single nucleotide variant rs6443265 (T>C) of the human OGG1 gene by polymerase chain reaction method in real time consists in using: forward primer OGG1-F 5'-AGAGACTTGTTCGGGTTGCT-3', reverse primer OGG1-R 5'-GGGAATCCATCACAGTGCCT-3', OGG1-T-allele-specific fluorescent-labelled probe 5′-FAM-ATGTCACCTATACCCTT-RTQ1-3′, OGG1-C-allele-specific fluorescent-labelled probe 5′-ROX-ATGTCACCTATACCCTC-BHQ-3′.

EFFECT: simplifies method of human genotyping by alleles rs6443265-T and rs6443265-C of the OGG1 gene, reduces analysis time.

1 cl, 2 dwg, 3 ex

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RU 2 831 204 C1

Authors

Azarova Iuliia Eduardovna

Semikina Elena Viktorovna

Klesova Elena Iurevna

Polonikov Aleksei Valerevich

Dates

2024-12-02—Published

2024-07-04—Filed