METHOD FOR GENOTYPING SINGLE-NUCLEOTIDE VARIANT rs2097461 (T>C) OF HUMAN XBP1 GENE BY REAL-TIME POLYMERASE CHAIN REACTION Russian patent published in 2024 - IPC C12Q1/68 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, particularly to a method for genotyping a single nucleotide variant rs2097461 (T>C) of the human XBP1 gene. Said method is carried out by a real-time polymerase chain reaction and a forward primer XBP1-F, reverse primer XBP1, as well as XBP1-T- and C-allele-specific fluorescent-labelled probes are used, wherein for allele T homozygous DNA samples with genotype rs2097461-T/T fluorescence increase in FAM channel is detected, for allele C homozygous DNA samples with genotype rs2097461-C/C signal is detected by channel ROX, for heterozygous samples with genotype rs2097461-T/C there is increase of fluorescence by both channels of detection.

EFFECT: present invention provides a simple, fast and cost-effective method of genotyping a single nucleotide variant rs2097461 (T>C) of the human XBP1 gene by real-time polymerase chain reaction.

1 cl, 2 dwg, 3 ex