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Application

4148925/13, 1986-07-31

(22)

Actual filing date

1986-07-31

(45)

Published

1994-12-30

(72)

Inventor

Ovchinnikov Ju.A.Sverdlov E.D.Tsarev S.A.Rokhlina T.O.Azhikina T.L.Arsenjan S.G.Kusov Ju.Ju.Grabko V.I.Nastashenko T.A.Balajan M.S.Drozdov S.G.Chizhikov V.E.Petrov N.A.Vasilenko S.K.Prikhod'Ko G.G.

RECOMBINANT PLASMID DNA PUR 292-HAV 23 ENCODING SYNTHESIS OF HYBRID POLYPEPTIDE OF HEPATITIS VIRUS A ANTIGENIC DETERMINANTS WITH β-GALACTOSIDASE Russian patent published in 1994 - IPC

Abstract SU 1380211 A1

FIELD: genetic engineering. SUBSTANCE: new recombinant plasmid pUR 292-HAV 23 consists of Pst-linearized plasmid pUR 292 which is a derivative of plasmid pBR 322 and contains promoter, operator and structural gene of complete b-galactosidase, and genome sequence of hepatitis virus A with length 3372 base pairs encoding permanent polypeptide which contains protein VP4 part (38 amino acids), proteins VP2, VP3, VP1 and certain nonstructural proteins (by 1167 amino acids). New recombinant plasmid provides expression antigenic determinants of hepatitis virus A in E. coli cells which are able to induce antibody formation in animals binding native virus. EFFECT: preparing of new recombinant plasmid indicated above. 1 dwg, 1 tbl

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SU 1 380 211 A1

Authors

Ovchinnikov Ju.A.

Sverdlov E.D.

Tsarev S.A.

Rokhlina T.O.

Azhikina T.L.

Arsenjan S.G.

Kusov Ju.Ju.

Grabko V.I.

Nastashenko T.A.

Balajan M.S.

Drozdov S.G.

Chizhikov V.E.

Petrov N.A.

Vasilenko S.K.

Prikhod'Ko G.G.

Dates

1994-12-30—Published

1986-07-31—Filed

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