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2 151 801

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Application

98114360/13, 1998-07-28

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Start date

1998-07-28

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Actual filing date

1998-07-28

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Published

2000-06-27

(72)

Inventor

Susloparov M.A.

(73)

Holder

Gosudarstvennyj Nauchnyj Tsentr Virusologii I Biotekhnologii

RECOMBINANT PLASMID DNA PUL 32 HCMV DETERMINING EXPRESSION OF HUMAN CYTOMEGALOVIRUS GENE UL 32 FRAGMENT ENCODING HYDROPHILIC SITE OF BASIC MATRIX PROTEIN PP 150 IN ESCHERICHIA COLI BACTERIUM CELLS Russian patent published in 2000 - IPC

Abstract RU 2151801 C1

FIELD: biotechnology, genetic engineering, molecular biology. SUBSTANCE: recombinant plasmid DNA has human cytomegalovirus gene UL 32 fragment that encodes the total hydrophilic site (3 antigenic epitopes) of basic matrix phosphoprotein pp 150 and 3'-end located DNA fragment that encodes polyhistidine tract used for purification of protein by affinity chromatography method. Structure provides effective biosynthesis of polypeptide. Obtained recombinant polypeptide shows antigenic properties of human cytomegalo-virus. The use of this antigen in immunoenzyme assay for detection of cytomegalovirus-specific immunoglobulins in patient blood shows 98-99% of sensitivity and specificity for IgG-human cytomegalovirus and 80-90% for IgM-human cytomegalo-virus. EFFECT: valuable antigenic properties of antigen. 4 dwg, 2 ex

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RU 2 151 801 C1

Authors

Susloparov M.A.

Dates

2000-06-27—Published

1998-07-28—Filed

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