FIELD: microbiology, biotechnology. SUBSTANCE: construction of vector plasmid pIL207 is based on replacement of smaller Bam HI-Sal Gi-fragment of pBR322 plasmid with Bam HI-Sal GI-fragment of DNA plasmid pILL 435 containing immunity region of lambda phage. The presence of plasmid pILL 435-fragment containing early promoters of λ-phage and gene-regulators: CI with thermosensitive mutation and cro-gene with suppressible amber-mutation provides effectiveness of vector pIL 207 with regard to regulated expression of foreign genes. Method ensures to prepare plasmid pIL207 containing necessary components for regulated expression of foreign genes and restriction sites, which are available for insertion of genes under control of early promoters of lambda phage PL and PR. EFFECT: construction of plasmid vector indicated above. 3 cl
