FIELD: medicine. SUBSTANCE: antiserum is incubated with affinity sorbent containing thyroid hormone. Concentration of latter exceeds the maximal binding capacity of antibodies in antiserum by 5-20-times. Then washing is carried out with solution of NaOH at pH 10,0-12,0 following by antibodies desorption with 15- 50% aqueous solution of acetonitrile at pH 2,0-4,0. EFFECT: simplified method, enhanced affinity of antithyroid antibodies. 2 tbl
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Authors
Dates
1994-01-15—Published
1987-04-03—Filed