STRAIN OF CULTURED SHEEP EMBRYO CELLS FOR PROPAGATION OF VIRUSES Russian patent published in 1994 - IPC

FIELD: virusology. SUBSTANCE: strain 4184 is based on strain of diploid cells 41. The medium is mixture of equal volumes of 0.3-% lactalbumin hydrolyzate and Eagle's solution (pH 7.0) with 5% of bovine serum. Heteroploid strain 4184 shows stability in morphological and biological characteristics. The species identity of the strain is confirmed by karyologic and isoenzymatic analysis. A bank of cells for inoculation has been created at the 45th passage level. The bank has 215 ampules with 6 10 cells in every ampule. This pool has been checked for safety. There are also 1.5 billion cells at different passage levels in large containers. The strain is free from foreign agents such as microbes, fungi, micoplasma, viruses. The strain is not carcinogenic, and it meets all WHO standards. Strain 4184 is sensitive to viruses of distemper, measles, Japanese encephalitis and various enteroviruses. EFFECT: safety and convenience in use.