Show metadata Hide metadata

(19)

(11)

1 839 446

(13)

(51)

IPC

(21) (22)

Application

4838187/13, 1990-05-07

(22)

Actual filing date

1990-05-07

(45)

Published

1996-04-27

(72)

Inventor

Leonova E.B.Gavrjuchenkova L.P.Gromova O.A.Khrushcheva T.A.Tsukanova L.N.Korshunov M.A.Mikhlin V.S.Melekhov V.M.Beljaev S.V.Vul'Fson A.N.Mal'Tsev K.V.Kulikov S.V.Morozov S.M.

METHOD FOR PURIFICATION OF RECOMBINATE PROTEIN WHICH CONTAINS PROINSULIN SEQUENCE Russian patent published in 1996 - IPC

Abstract SU 1839446 A1

FIELD: biotechnology. SUBSTANCE: method is carried out by chromatographic purification on anionite. Chromatography takes place on copolymer of ethylene glycol dimethylacrylate with 2-hydroxy-3-diethylaminopropyl methacrylate, 0.007-0.013 M acetic acid which contains 0.15-0.30 M of sodium acetate being used. Protein is eluted by 0.8-1.1 M acetic acid. Yield of protein is up to 73 %. EFFECT: increases yield of desired product. 2 tbl

Similar patents SU1839446A1

Title	Year	Author	Number
METHOD FOR ISOLATION OF RECOMBINANT PROINSULIN	1991	Leonova E.B. Gavrjuchenkova L.P. Gromova O.A. Khrushcheva T.A. Tsukanova L.N. Korshunov M.A. Melekhov V.M. Mikhlin V.S. Beljaev S.V. Vul'Fson A.N. Mal'Tsev K.V. Kulikov S.V.	RU2049793C1
PROCESS FOR PREPARING S-SULFONATE	1991	Leonova E.B. Gavrjuchenkova L.P. Gromova O.A. Khrushcheva T.A. Tsukanova L.N. Korshunov M.A. Mikhlin V.S. Melekhov V.M. Beljaev S.V. Vul'Fson A.N. Mal'Tsev K.V. Kulikov S.V.	RU2025474C1
METHOD OF ISOLATION OF HUMAN CU,ZN-SUPEROXIDE DISMUTASE	1994	Solov'Eva L.Ja. Sharonova V.L. Churilova I.V. Khrushcheva T.A. Fedorova N.M.	RU2111754C1
PROCESS FOR PREPARING HUMAN PROINSULIN	1991	Mal'Tsev K.V. Vul'Fson A.N. Ivanov V.T.	RU2045535C1
METHOD OF PREPARATION OF RECOMBINANT ANTAGONIST OF HUMAN INTERLEUKINE-1 RECEPTOR "ARIL"	2004	Antipova Tat'Jana Olegovna Antropova Galina Fedorovna Ishchenko Aleksandr Mitrofanovich Ketlinskij Sergej Aleksandrovich Mitrofanov Evgenij Vital'Evich Pigareva Natalija Vasil'Evna Poljakova Elena Akimovna Protasov Evgenij Aleksandrovich Sventitskij Evgenij Nikolaevich Simbirtsev Andrej Semenovich Solov'Eva Ljudmila Jakovlevna Frolov Vladimir Nikolaevich Shalaeva Ol'Ga Nikolaevna	RU2326947C2
METHOD OF ISOLATION AND PURIFICATION OF INSULIN OR ITS BIOTECHNOLOGICAL PRECURSORS	1994	Mal'Tsev K.V. Miroshnikov A.I. Vul'Fson A.N. Gavrjuchenkova L.P. Gromova O.A.	RU2081122C1
METHOD OF RECOMBINANT HUMAN INSULIN PREPARING	1999	Kalinin Ju.T. Urakov N.N. Ivanov V.T. Stepanov A.V. Miroshnikov A.I. Gavrikov V.G. Bairamashvili D.I. Donetskij I.A. Korobko V.G. Kostromina T.I. Guljaev V.A. Litvinenko M.A. Bajdus' A.N. Korobov V.I. Krasil'Nikov V.A. Borisov N.V. Shmatchenko N.A. Vorotnikova I.I. Gorkun O.G. Derbyshev V.I. Nozdrin V.N. Dunajtsev I.A. Lipin M.Ju. Bekmuratova I.I. Kobeleva V.A. Shepelin A.P. Martovetskij M.N. Fedjukin V.S. Abrosimova L.I.	RU2141531C1
METHOD FOR INDUSTRIAL PREPARING HUMAN RECOMBINANT INSULIN	2003	Kalinin Ju.T. Stepanov A.V. Bajdus' A.N. Nozdrin V.N. Aitov R.S. Kolesnichenko I.F. Shmatchenko N.A. Babaev M.A. Borisov N.V. Krasil'Nikov V.A. Gorkun O.G. Astashkina G.F. Ushakova N.I.	RU2232813C1
METHOD OF ISOLATION OF FUSED PROTEIN CONTAINING HUMAN PROINSULIN AMINO ACID SEQUENCE	1991	Mal'Tsev Konstantin Valentinovich[Ru] Vul'Fson Andrej Nikolaevich[Ru] Murav'Eva Tat'Jana Igorevna[Ru] Kovalenko Viktor Andreevich[Ru] Galiev Alik Khazievich[Kz] Kharitonov Vladimir Grigor'Evich[Kz] Petrov Pavel Petrovich[Kz] Ivanov Vadim Tikhonovich[Ru]	RU2067100C1
METHOD FOR PREPARING HIGH-PURITY CRYSTALLINE INSULIN OF ANY ORIGIN	2011	Jaromir Klanchik	RU2453331C1

SU 1 839 446 A1

Authors

Leonova E.B.

Gavrjuchenkova L.P.

Gromova O.A.

Khrushcheva T.A.

Tsukanova L.N.

Korshunov M.A.

Mikhlin V.S.

Melekhov V.M.

Beljaev S.V.

Vul'Fson A.N.

Mal'Tsev K.V.

Kulikov S.V.

Morozov S.M.

Dates

1996-04-27—Published

1990-05-07—Filed