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2 081 122

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IPC

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Application

94023134/13, 1994-06-16

(22)

Actual filing date

1994-06-16

(45)

Published

1997-06-10

(72)

Inventor

Mal'Tsev K.V.Miroshnikov A.I.Vul'Fson A.N.Gavrjuchenkova L.P.Gromova O.A.

(73)

Holder

Institut Bioorganicheskoj Khimii Im.M.M.Shemjakina I Ju.A.Ovchinnikova Ran

METHOD OF ISOLATION AND PURIFICATION OF INSULIN OR ITS BIOTECHNOLOGICAL PRECURSORS Russian patent published in 1997 - IPC

Abstract RU 2081122 C1

FIELD: biotechnology, biochemistry. SUBSTANCE: solution containing insulin or its biotechnological precursor is subjected for chromatography on cation-exchange resin based on copolymer of methacrylate monomer with cross-agent at pH = 4.5-5.8. Proteins bound with resin were eluted at salt gradient and fractions containing the end product were desalted and subjected for chromatography on anion-exchange resin based on copolymer of methacryalte monomer with cross-agent containing quaternary amino-groups, at pH = 9.0-10.5. Preferable cation-exchange resin is sorbent based on copolymer of methacrylate monomer with cross-agent modified with phospho-groups. Desalting is carried out by ultrafiltration. Purity degree of insulin or its biotechnological precursors - above 97.5%. EFFECT: improved method of isolation and purification, increased purity of product. 4 cl

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RU 2 081 122 C1

Authors

Mal'Tsev K.V.

Miroshnikov A.I.

Vul'Fson A.N.

Gavrjuchenkova L.P.

Gromova O.A.

Dates

1997-06-10—Published

1994-06-16—Filed

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