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2 326 947

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IPC

C12P21/02(2006-01-01)

C07K1/16(2006-01-01)

C12N15/12(2006-01-01)

(21) (22)

Application

2004134028/13, 2004-11-23

(24)

Start date

2004-11-23

(22)

Actual filing date

2004-11-23

(45)

Published

2008-06-20

(72)

Inventor

Antipova Tat'Jana OlegovnaAntropova Galina FedorovnaIshchenko Aleksandr MitrofanovichKetlinskij Sergej AleksandrovichMitrofanov Evgenij Vital'EvichPigareva Natalija Vasil'EvnaPoljakova Elena AkimovnaProtasov Evgenij AleksandrovichSventitskij Evgenij NikolaevichSimbirtsev Andrej SemenovichSolov'Eva Ljudmila JakovlevnaFrolov Vladimir NikolaevichShalaeva Ol'Ga Nikolaevna

(73)

Holder

Obshchestvo S Ogranichennoj Otvetstvennost'Ju

METHOD OF PREPARATION OF RECOMBINANT ANTAGONIST OF HUMAN INTERLEUKINE-1 RECEPTOR "ARIL" Russian patent published in 2008 - IPC C12P21/02 C07K1/16 C12N15/12

Abstract RU 2326947 C2

FIELD: technological processes; pharmacology.

SUBSTANCE: antagonist of human interleukine-1 receptor is prepared with the help of recombinant strain E. coli that contains plasmid, which provides production of this protein. For this purpose the strain is cultivated. Then from bacterial cells the target product is separated with application of three-stage chromatography and concentration on hydrophobic sorbent. At that the first and the third stage of mentioned chromatographic purification is performed on cation-exchanging resin, and in the second stage of chromatography the anion-exchanging resin is used.

EFFECT: application of invention allows to prepare antagonist of human receptor of high purity.

7 cl, 1 tbl, 4 ex

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RU 2 326 947 C2

Authors

Antipova Tat'Jana Olegovna

Antropova Galina Fedorovna

Ishchenko Aleksandr Mitrofanovich

Ketlinskij Sergej Aleksandrovich

Mitrofanov Evgenij Vital'Evich

Pigareva Natalija Vasil'Evna

Poljakova Elena Akimovna

Protasov Evgenij Aleksandrovich

Sventitskij Evgenij Nikolaevich

Simbirtsev Andrej Semenovich

Solov'Eva Ljudmila Jakovlevna

Frolov Vladimir Nikolaevich

Shalaeva Ol'Ga Nikolaevna

Dates

2008-06-20—Published

2004-11-23—Filed