METHOD OF COLLAGENASE PREPARATION PRODUCING Russian patent published in 1994 - IPC

Abstract RU 2008353 C1

FIELD: biochemistry, enzymology. SUBSTANCE: collagenase from crab hepatopancreas is isolated by homogenization, separation of supernatant by centrifugation and precipitation of enzyme with ammonium sulfate. Then ion-exchange chromatography on aminated silicate materials is carried out at pH 5.2-6.4, and collagenase is eluted with buffer solution containing 1M NaCl and 15-25% isopropanol, pH 7.0-8.5. Enzyme is isolated from eluate at saturation with ammonium sulfate up to 60% . Yield of collagenase is at 40-fold purification degree. EFFECT: improved method of collagenase isolation. 2 cl, 1 tbl

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RU 2 008 353 C1

Authors

Isaev V.A.

Rudenskaja G.N.

Kupenko O.G.

Stepanov V.M.

Popova I.M.

Didenko Ju.G.

Dates

1994-02-28—Published

1991-08-05—Filed