FIELD: biochemistry, biotechnology. SUBSTANCE: bovine serum albumin (BSA) is isolated from blood plasma by precipitation with ethanol, by change of pH and ionic strength. Proteolysis of extrinsic proteins is carried out additionally with trypsin in bicarbonate buffer, pH 7.8. BSA is separated by gel-filtration on Sephadex G-75. EFFECT: improved method of purification. 1 dwg
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