FIELD: medicine; pharmaceuticals.
SUBSTANCE: invention relates to medicine and pharmaceutics, namely to a method for sensibilization of a plate for enzyme immunoassay by insoluble protein antigens of a plague microbe, involving destruction of bacterial mass by ultrasound followed by separation of subfractions, affinity chromatography of proteins, separation and dissolution of purified target protein precipitate with denaturation of urea and determination of their concentration in solutions, characterized by that a solution of denatured in purified urea fibres MetQ, OmpA, Fba and YapM is added to the wells of the plate and subsequent ten-fold dilution in the wells with a carbonate-bicarbonate buffer solution, accompanied by renaturation of proteins and their passive immobilisation in polystyrene plates.
EFFECT: technical effect consists in sensitizing polystyrene plates with protein antigens, insoluble in standard buffer systems used at the first stage of treatment, and performing ELISA for such protein antigens, thereby increasing sensitivity and specificity of the test.
1 cl, 5 dwg, 1 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR RENATURED PROTEIN G ISOLATION FROM MARKED POLYACRYLAMIDE GEL | 2016 |
|
RU2646103C2 |
| DIAGNOSTIC TECHNIQUE FOR RICKETTSIAL DISEASES GROUP OF SPOTTED FEVER, IMMUNOASSAY DIAGNOSTIC TEST SYSTEM FOR IMPLEMENTATION THEREOF | 2019 |
|
RU2726484C1 |
| METHOD OF DETERMINING HIGH LEVEL OF AUTOANTIBODIES TO HUMAN PLASMINOGEN AND DEGRADATION PRODUCTS THEREOF AND DIAGNOSTIC TEST SYSTEM THEREFOR | 2014 |
|
RU2597783C2 |
| METHOD OF DIFFERENTIATING VIRULENT LISTERIA MONOCYTOGENES STRAINS FROM AVIRULENT STRAINS USING POLYCLONAL ANTIBODIES AGAINST PATHOGENICITY FACTORS INTERNALIN A (INLA) AND INTERNALIN B (INLB) | 2023 |
|
RU2808590C1 |
| METHOD OF DETERMINING CONTENT OF PROTEOLYSIS PRODUCTS IN BLOOD PLASMA AND DIAGNOSTIC TEST SYSTEM THEREFOR | 2015 |
|
RU2597782C1 |
| METHOD OF PRODUCING RECOMBINANT HUMAN GLIAL FIBRILLARY ACIDIC PROTEIN AND THE PRODUCER STRAIN USED IN IT | 2022 |
|
RU2811427C1 |
| SET FOR QUANTITATIVE DETERMINATION OF AVERMECTINS VIA SINGLE-STEP COMPETITIVE ENZYME-IMMUNOASSAY | 2009 |
|
RU2416094C1 |
| METHOD OF PREPARING IMMUNOGENIC COMPOSITION BASED ON THREE FUSION PROTEINS OF SHELL OF TICK-BORNE ENCEPHALITIS VIRUS DEFINING BELONGING TO SIBERIAN (DBD2-D3S), EUROPEAN (DBD2-D3E) AND FAR EAST (DBD2-D3D) VIRUS SUBTYPES; RECOMBINANT PLASMIDS pDBD2-D3S, pDBD2-D3E AND pDBD2-D3D; PRODUCER STRAINS Escherichia coli M15 [pREP4]; CHIMERIC PROTEINS AND THEIR APPLICATION | 2014 |
|
RU2560588C1 |
| METHOD FOR IMMUNOFLUORESCENT DETECTION OF PROTECTIVE ANTIGEN OF ANTHRAX AGENT | 2011 |
|
RU2478970C1 |
| ANTIBODIES AGAINST HUMAN STABILIN-1 RECEPTOR | 2023 |
|
RU2823309C1 |
