FIELD: biotechnology, medicine. SUBSTANCE: method of insulin preparing from pancreas gland involves sorption chromatography using carriers on the basis of silica gel and macroporous glass. Insulin isolation from the spirituous extract is carried out by 4 stages, purification involves 2-3 stages. By electrophoresis data in polyacrylamide gel prepared insulin does not contain practically impurity proteins (less 0.1%). Yield is 25 mg from 1 l spirituous extract. EFFECT: simplified method, low cost. 2 cl
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