FIELD: medicine.
SUBSTANCE: strain Escherichia coli Russian National Collection of Industrial Microorganisms B-10996 is cultivated on a nutrient conditioned medium containing: peptone 2-4 wt %, yeast extract 1 - 2 wt % and water the rest in the presence of a selective antibiotic to stationary growth phase at temperature 20 °C to 30°C. The prepared is dissolved in a fresh portion of the same medium in the ratio 1:1 to 1:3. Simultaneously an inducer is added, and the cultivation is continued to for at least 4 hours. Synthesised proteinase is purified.
EFFECT: ensured preparation of enzymatic active proteinase U1p275 - a version of SUMO-proteinase U1p1 of yeast Saccharomyces cerevisiae which contains a catalytic domain of proteinase U1p1 and comprises a sequence of 6 histidine residues on the C-terminal.
9 ex
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