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2 153 535

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IPC

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Application

99109717/13, 1999-05-14

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Start date

1999-05-14

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Actual filing date

1999-05-14

(45)

Published

2000-07-27

(72)

Inventor

Gul'Ko L.B.Okorokova N.A.D'Jakov N.A.Sokolov A.K.Vejko V.P.Debabov V.G.

(73)

Holder

Gosudarstvennyj Nauchno-Issledovatel'Skij Institut Genetiki I Selektsii Promyshlennykh Mikroorganizmov

RECOMBINANT PLASMID DNA PSA V27 ENCODING SYNTHESIS OF SOLUBLE STREPTAVIDINE FROM STREPTOMYCES AVIDINII AND BACTERIAL STRAIN ESCHERICHIA COLI AS PRODUCER OF SOLUBLE STREPTAVIDINE FROM STREPTOMYCES AVIDINII Russian patent published in 2000 - IPC

Abstract RU 2153535 C1

FIELD: molecular biology, microbiological industry, medicinal biotechnology, genetic engineering. SUBSTANCE: invention proposes recombinant plasmid DNA pSA V27 encoding synthesis of soluble streptavidine secreted into periplasm and used as the base for construction of the strain Escherichia coli VKPM B-7758 as a producer of streptavidine. Strain accumulates 180-240 mg/l of streptavidine in cultural fluid for 1-14 h of fermentation. EFFECT: recombinant plasmid indicated above, increased yield of streptavidine. 3 cl, 3 dwg, 4 ex

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RU 2 153 535 C1

Authors

Gul'Ko L.B.

Okorokova N.A.

D'Jakov N.A.

Sokolov A.K.

Vejko V.P.

Debabov V.G.

Dates

2000-07-27—Published

1999-05-14—Filed