FIELD: medicine. SUBSTANCE: strain of Candida tropicalis is cultivated in fermenter in cyclic regime. The process is carried out in liquid phase at pH 5.5, saturation by oxygen pO2 being at least 80 %, glucose concentration in said medium being 0.6-0.8 %. Thus prepared culture liquid is separated and concentrated. The process is followed by additional separation with the help of membrane filtration. Thus obtained allergen-containing material having molecular weight 100-300 kD is then fractionated, it may be used as diagnostic agent. EFFECT: improves efficiency of the method.
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Authors
Dates
1995-03-27—Published
1991-12-24—Filed